FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1987_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAATAAGTAAGCCATCAAGTAGATCCAGACTCAGTTCTTGTGTAGT17.142857142857142No Hit
CATGGTTTCACCTGTGAATTCTATGAATCATCCAAAGAAAAAAAATGAAT17.142857142857142No Hit
CCCTGCTGTCCCTGCAGCAGAGTCTCCAATTCCAGGATGCCAGCAAGGAG17.142857142857142No Hit
TCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTT17.142857142857142No Hit
CACTAAGACTTGAAAGAGCAGCAGTGTTAGGGGAAAGACTAGGGCCTCTA17.142857142857142No Hit
GAACAAAGCTGATAGAGTCTCAGCCAGATTCAAAGAAAGAGGACTCATCA17.142857142857142No Hit
GCGCTGGTTGTTGTCACCACACGCTGTCAGCGCCGGGACGTTTTCACACT17.142857142857142No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA17.142857142857142No Hit
CCAGTAGAGAGCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTC17.142857142857142No Hit
TGTCCCAGGGCCGCCCACTCGGCCTCCCTCCCTCGGGGGACTCCACAGAA17.142857142857142No Hit
CTCCTGATCAGTTGCCTTGGCTGTTTCTACCCTACAGCCACAGAGCCTGA17.142857142857142No Hit
GCAAGGAGCCCAATGTGGGGACTCGATCCCAGGACTCTGGGATCATGACT17.142857142857142No Hit
GGGCTATATGGAGCAATTGAGGAGAGCCAGATTAATGATCCCTGGCTTTT17.142857142857142No Hit
GCAGGGAGCCCCACACAGGGCTCGATCTCAGGACCATGGGATCATGACCT17.142857142857142No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)