FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1987-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905696
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC4100.051.2858623
GAGCGAA450.00962855548.34641
GCGAAAG4500.046.7271162
AGCGAAA4500.045.123311
TATACTA1502.5966074E-838.670725
GTGATCG1502.599154E-838.6664479
TATACTG3650.033.770665
TTAGGTA3100.032.745364
CATCGGT900.00362325132.2309341
CTAGTAT1153.1599248E-431.525043
GTAATAT3850.028.254391
GTATAAG2808.1709E-925.8998581
TATAATA9650.025.5467172
ACTGTGC5650.024.380398
CCGGTTG1500.001494213824.16653145
TGTACCG4053.274181E-1123.2740445
GTATTAT7550.023.052591
AAGGCGC1600.002173669622.658626
GGTTAGT1650.002600758621.9695729
ATACAGC6950.021.908776
Produced by FastQC (version 0.11.5)