FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1987-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219747
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1703.0029205E-629.8650571
GCGAAAG4000.027.1985341
CGACCCG1100.00967260326.363523145
AGCGAAA3601.8371793E-1024.1764761
GCTATAT4251.8189894E-1223.8920461
GCCTATA3203.3725883E-822.6654451
CGAAAGC4557.2759576E-1222.3085122
ATATAGG2353.6984442E-521.6045111
AGACGGT3055.342372E-721.393353145
TATATAC9900.021.2381533
GCGTGCG1750.003659274220.71429
GTATATC3551.0108488E-720.4308241
GATATAG3751.8012724E-719.3411791
GTGCGAT1900.005888757819.0788659
ATATACC6201.8189894E-1218.7103654
CTTATAT6600.018.6818221
GTAATAT4303.725654E-818.5540391
TAGGTAG5301.1787051E-917.7836745
GTATAAT4506.267874E-817.7294141
GTTGACA8350.017.3723161
Produced by FastQC (version 0.11.5)