FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1987-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965469
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23510.11961521652084058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3800.034.3455731
TACGGCC1453.49211E-529.9999774
TAGGGCG1306.485222E-427.8845965
GTGATCG2507.838935E-826.0999819
GTAGTAC1701.0317941E-425.5882173
TATACGG1751.256335E-424.8571245
TAGTACT5400.024.1666474
TTACGTG5450.023.9449372
GTCGTCC1902.1927147E-422.8970491
TACTATA5700.022.8947222
GTATTAT23500.022.5235541
CTAGTAC4256.184564E-1122.1764563
GTTATAC4607.2759576E-1222.06523
TAGCATG24450.021.6462022
GTTGACA20900.021.5093521
CTACATC10500.020.7142723
CGTGTTG6750.020.4073935
ATATACG2155.058548E-420.2325444
GTATAAT7900.020.1919161
GCATGTT28000.019.16074
Produced by FastQC (version 0.11.5)