FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1987-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246953
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4050.039.3872032
TACGGTG1051.852737E-434.5277445
CGAAAGC5250.033.1466333
AGCGAAA4650.032.752231
GTCTAAC2601.1417433E-725.1040481
TAGAGTG5150.023.9347655
GTACATA5000.023.2072961
ATTATAC3203.3831384E-822.6588333
CTTACGG1600.002174008922.6588333
CTACGGT1650.002599496921.97224
TAACACG2556.953147E-519.9042284
TAGGACT10550.019.2438984
TATACTG4553.430614E-919.1230585
TATATAC4202.85454E-818.990263
GTATTAG7300.018.87581
TTAGACT7300.018.8720134
TCTATAC13400.017.8565123
GTATAAT3753.8528196E-617.4054721
TAGAGGT6805.456968E-1217.0607664
GTCCTAT11300.016.6866631
Produced by FastQC (version 0.11.5)