FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1987-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1987-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022226
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC12520.12247780823418696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG657.336722E-444.61531145
GTATCCG2050.042.4389539
GTATATA4450.039.108711
GATCGAC750.001481987938.6741681
GCGAAAG5450.035.919132
CGAAAGC6800.028.7867163
GTATAGG4600.026.7986761
GTATAGA6800.026.6595841
AGCGAAA7100.026.5544451
CCGCTAT1701.0313264E-425.5881929
ATACCCC1701.0313264E-425.5881924
TACTGCG2301.1031734E-625.2173467
ATAATAC13300.024.5300333
TAATACT3952.3646862E-1123.860724
ATAGGTG6200.023.3870563
ACGTCCG1902.1932405E-422.89479
GTACCTA1600.00217520422.6562126
GTACGTA1600.00217520422.6562129
ACTGTCG2603.204328E-622.3076558
ATTGGAC2303.142153E-522.0651783
Produced by FastQC (version 0.11.5)