FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1986_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1986_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCAGGGACCTCAGGTGAGCAGAATCTTACATATTCACCCTCCTGTGA15.88235294117647No Hit
GTAATCACTCAGGATACACATATAAAACATATCAGCTAAATTCCTTCCTC15.88235294117647No Hit
ATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGTAATT15.88235294117647No Hit
GTTCTCATATTTTCCTTTTCGGTGCCTCTGCCATTGAGCTATGTCTCACG15.88235294117647No Hit
GTTTCACCTGTGAATTCTACGAATCATCCACAGAAAAAAATGAAAACCAA15.88235294117647No Hit
ATTGTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCT15.88235294117647No Hit
GGAGCAGAACAAGAAAAAATCCAAACTCTGTAACAAGAGCCAATGAACGG15.88235294117647No Hit
GATGAGACACTGCGCGCCGATCCCCCGCGGCCTCCCGCCTGCTGATGGCG15.88235294117647No Hit
AGACTTGGAACATAGAAACTTAAAGAAAATCTTGGCCAAAGTGCCGTCCC15.88235294117647No Hit
TTATATACCTGCTCCATGTAGACTCCTGCTCTGCTGGAATGGTGCAGACT15.88235294117647No Hit
AGAGGTATTATTTGCTTGTTTCTTTGTTTGTGGCAAAGGCCAGTTTGAAA15.88235294117647No Hit
CAAGAATACTACATAGCAACCATGGTTGGGAGCAGAAAGTAATGAGCAAA15.88235294117647No Hit
GGCTTGGGCAATTCTCTTGTGCTCAGTCACTAGCTGGAACAGCCAGACCT15.88235294117647No Hit
ACATTCTACTGAAAAACCAATTGGGTTAATTGTAAAACCAAAATAGGAAA15.88235294117647No Hit
GAACAATAACAGATCAACAAAGAGATGGTTCAATGAAAAGAACAAACACG15.88235294117647No Hit
CCCATCATTCTCTTCTGGTCTGTAGAGGCGAAAGCCCTGTGAGGGCAAGA15.88235294117647No Hit
AGGAAGGCCTGTCCTGTGCCTCTGGTCCCAAGGGGCAGCAGCTCCTTGGC15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)