FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1986-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1986-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292448
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC38800.30020550149793257No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT33730.26097761766817695No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC31200.2414023620292654No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA27540.21308400802198618No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG25770.1993890663299413No Hit
GTTCTGGACCCTCCCTTCTCTCCTCCAGGCCCAGGTTCCGGCTCCCGCTT25350.19613941914877814No Hit
GATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCT24510.18964012478645176No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA22040.1705291044591349No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT21070.1630239669216866No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG20520.1587684765654015No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20160.15598306469583303No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC19940.154280868553319No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG18240.1411275347248013No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT17570.1359435737453267No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA14440.11172596499046769No Hit
ATGTAGGACTGTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACAGA14330.11087486691921068No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT13750.10638725890712818No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA13660.10569090593973607No Hit
CCTCAAGACCATGAGAAGTCCCTGCTGGCTCCCTTCCTGAAGGTTCTTCT13640.10553616083587115No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG13600.10522667062814131No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC13430.10391133724528955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG2850.089.041575
CGAAAGC4300.079.2499543
GCGAAAG4450.076.578612
GACAGTA4850.064.278067
GTAATAC1150.063.048072
GGTAATA1200.060.4327621
ATTATAC1750.058.0042233
TTTCGTT502.0105104E-457.999737145
GCCGAGT400.00605139454.3747528
GGACCGT400.00605139454.3747529
TAACTAT2200.052.7413181
GGATAGC8150.051.5988465
GACAATC5750.050.434557
AGTCTCG604.9457327E-448.3331158
ACCCCGG1351.9099389E-1048.333115145
AGTAGAA16350.047.9026641
GTACTGA5650.047.481346
TATACAT2500.046.403385
AGGTACT5950.046.3058934
CAGGTAC5600.045.31583
Produced by FastQC (version 0.11.5)