FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1986-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1986-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473383
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC70630.4793729804131037No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG53390.3623633501947559No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT40530.27508122463745No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG34520.2342907444975271No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA33680.228589579220067No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA30770.2088391137945802No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA28190.19132839187095277No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT26550.180197545376864No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC24800.16832011771548877No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC23770.16132940314907937No Hit
CCTTCCCGATTGAGCCTTCCTCCACTCCCTTAGGTGACTCTCCAATTGGC23480.1593611437080515No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC22910.15549249584120353No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG21650.1469407479250134No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG20690.140425130465059No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC20470.1389319681304861No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT20190.13703157970466606No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA18790.1275296375755659No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC18500.125561378134538No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG18360.12461118392162798No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC18290.12413608681517296No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC18190.12345737666309439No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA18110.12291440854143153No Hit
CCTTTACAGTTACCGGAGACAACACCAAATGGAATGAGAATCAGAATCCT17840.12108189113081934No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA17610.1195208577810386No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA17090.11599156499022996No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG17060.1157879519446064No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC17000.11538072585335925No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG16770.11381969250357848No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT16110.10934020549985984No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT16000.10859362433257341No Hit
CAGATACACCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGAA15940.10818639824132625No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT15890.10784704316528697No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA15500.10520007357218049No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG15090.10241736194865829No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT14960.10153503875095612No Hit
ATTATGTACTCAGTGGCCCTGCAATGAGACACTTCCGCTGTGAAGTAGTT14840.10072058656846183No Hit
GGTAAAGGCAGTCCGAGGTGATTTGAATTTCGTAAACAGAGCAAATCAAC14740.10004187641638326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTG351.902663E-9124.285286145
GTCCGTT256.4660107E-6115.999596145
GACTTAA458.54925E-11112.77738145
AGTAGAA3800.0110.6762851
AGCTAAA203.8664785E-4108.74962145
GGTACTG1850.0105.817615
GCAGGTA2100.0100.1254962
CGCAATT301.5997397E-596.66633145
GGGTAGA259.385236E-487.00566
CTCGGTT259.387767E-486.9997145
TAACTAT5200.069.723121
AAGGTAC1600.067.968519
AGAAACA6700.062.7652324
AGGGTAG350.003565928762.146865
TGGGCGG350.003566887762.142643145
GCTCCCC606.8073296E-660.416454145
GCGAAAG502.008907E-458.009641
TATAGAC753.762525E-757.9997987
ATACCAC502.0105929E-457.999798145
AACACTG5800.057.50375
Produced by FastQC (version 0.11.5)