FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1986-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1986-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057213
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAG2252.8174327E-828.999914145
TTATACG1050.007706979327.6202724
AACCGCG1050.00770875827.6189677
TATACGT1409.992386E-425.8940055
GTATATA4650.024.9508841
GCGCACG1751.2558173E-424.857069145
GTATTAT6100.023.774921
GTAATAC3854.274625E-1022.5984063
TACTATA3406.4294E-821.3244762
GCGAAAG5151.8189894E-1221.1174412
GTATAAT3851.0491931E-820.7181431
TATACCT4251.4697434E-920.4714955
GTACATC2205.9019064E-419.7736036
CTATACT4502.9922376E-919.3341924
CTATATC7200.019.1327934
CGAAAGC5759.094947E-1218.9138813
TATACTG3852.3842404E-718.8320055
TTTATAC8100.018.797133
TACTACG1950.006839059818.5905692
GTATAAG3703.39311E-617.638421
Produced by FastQC (version 0.11.5)