FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1986-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1986-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGGAAACCTTATTTGTTCTCAGTTCTGGTAACTCGCTCACTCTAC11.25No Hit
AGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTG11.25No Hit
GGAGATCTCTCACTGAGATGATTCCTTATTTATAAAAAGTCATTAACTTT11.25No Hit
GCAAAGGACTTGGGGGACATAAAGACAGGACCGCTGAGGGTCATCAGCCG11.25No Hit
GAAGTGGGAATGACAAGGAAGGACCAGCTGCGTGGAAATCTGGAGTACCA11.25No Hit
GTTGAGCTGCATGAGGTTCTTGAGGCCCTGGAAGGTGTCGGGCTTGAAGA11.25No Hit
GCTTGTGCACTCTCTTTCTGTTTCTACAATAAATAAGTATGTCTTTAAAA11.25No Hit
GGGTTGGGTAACTGCCGGATCCTGGGTGTTTCATCACTTGTTTTCCCCTG11.25No Hit
ATGCTATGCTTTCACAGAGCTTTTCAGCTTGGCGCGAGCCGCACATCGCT11.25No Hit
GAGTTCTTGGGGAGCCCCCGGTCTGACAGCCTGTTGTAGTTCAGGCGAAT11.25No Hit
GGGTTCTGTGTGTATCTGTCAAGTCCATCTAAGTAACAGGTTGTCTGAGG11.25No Hit
GCCCTGGCTGTCATTTTGAAAAGAGAGTGTAAGGAGCCAGGGCTTAAGTA11.25No Hit
CCTCCACACTGGGGCCCTCATGTTGTCAAGTTCTGTTGTCCCAAAAGCTA11.25No Hit
CATGAACCCTGGCCATGCAGATCTCAGCGCTAAAGAAGCACAAGATGTCA11.25No Hit
GAGAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATG11.25No Hit
GCTGAGGGAAGGTAAGGGTGCAGTTTGCTCCTGCAGGAGAAGTGGCCGGA11.25No Hit
CCAATACCATAGGGAACTTTAATACCTCACACTTCTCTCAATGATCCCCC11.25No Hit
ATTCAAAAATGAAACAAAAAAACAACAAAAAAAAAAAATAACTCAAAAAC11.25No Hit
GAATTGAGCCATTTCTGAAGACAACACCACGTCCCCTCAGATTGCCTGAT11.25No Hit
GTTTGGAGGTTGCCTGTAAGCACTTCTTCCTCTCTTTTGACAGAAGACCC11.25No Hit
GTTCATTGATGCTTAATGCTGATTCTTCTCCTGTCTTCCTGATGTGTACT11.25No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT11.25No Hit
CCGCTATGGGTTTGTAGCCAACTTCAGCATGGAGCTGCCCAGCTTTGGAG11.25No Hit
TTCCTGGACTGACCCAGCTAGCGCATGGCAGAGCCAAGACGTGATGTCAG11.25No Hit
CTCTTATAGAATACCATGATTTAACATTAATATGAATTACTACCTCTAAA11.25No Hit
GCCACCAGGCCGTGAGCGGCTTGTCAATGCTGAGCTGAATGCGGAGCAGG11.25No Hit
GAGGGAATGGGTCCAAGACCTGTGCGGCAGGTGCCCCCCACCCCAAAAAT11.25No Hit
AAAATTAAGTGAGCAATCTTACGATTGCGTTGAAAGCAAATCATGACACT11.25No Hit
GGCTGTGTTTTTGAAGTTCTGAGACTGTGTCTGTGCTTGTTTTTATTGTT11.25No Hit
GTTCTACGAAGCCGTTTCACGAAGTACCGGATAGAATGATTGCTCGCTGA11.25No Hit
TATATGTTCATTGGCTTTTGGTCCATCTTCACTACTAAGCTGAAAGCTTA11.25No Hit
GCAGGGGAGTTACCTCTCACAAGTATTCTCATTCCTGATCCCCTCACATT11.25No Hit
GCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGACCTCTGGAGTTAG11.25No Hit
GGGTAATGGTATCACCTCTGGCTGTAACGTGGTGGAACAGAAATGGACCA11.25No Hit
CAGATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGATAGAGTCT11.25No Hit
CTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGGTAGCTC11.25No Hit
TAACATGAGATCATGGATCAAATAATTGCAAATGAACCATTTAACACAAA11.25No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG11.25No Hit
GGAGAGAGGTAACCCCAGGTGGAGAGCTGAGGAGGAGCACAAGGACACAC11.25No Hit
GCACGAAATGTTCTACGAAGCCGTTTCACGAAGTACCGGATAGAATGATT11.25No Hit
CTACCTTATTTAGTTTGTAAACATAATTCAGAGTCAGTGTGGCTGTGTTT11.25No Hit
ATTGACACAGAGCTGGGGAGAGAGACAGACAGACAGACTGAGCAAAGTCG11.25No Hit
ACATTGAAGCAGGAAGGGTTTAACAGGCAAACACTTACATTTTAACAAAT11.25No Hit
TATTAACTTAATGAATAAACATAAATCACTTAATATAATAGTAATGACTT11.25No Hit
AAATAATACAAAGAAACAAAATTCACAGAATCCAGAAAAACAGTTTATAA11.25No Hit
GAATTTTCCCAAATGAGGACTGAAATCAAATCAACCATCTTTTCCAACCA11.25No Hit
CCCTGTAAGTGAGGCATATTTGCCAGATATAACCTGTAATCTTTGCTTTT11.25No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT11.25No Hit
CTTAATGACTGAGCCACCCTGGCGCCCCCAAACTGTGCTTTTTTTGTCAG11.25No Hit
GGCTTGAGGAAATGCTGTTTTTGCACTATGTGCACTATTTGTCGAGAAGA11.25No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT11.25No Hit
CAATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGAT11.25No Hit
GAAAGAAAGTGCCACAGGCATGCAGGCGTCATTTTATGGGTATGATGCTC11.25No Hit
GAATCTTCACTTGGGGACATGCATGAACCCAACCAAATACAGCCCAGGAA11.25No Hit
GCTTAGGACCCTATGGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAG11.25No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC11.25No Hit
GTCTTGATTGTATTTGTCTTTATGCCTCTGCAGTGGTAGGACGGTTCTCA11.25No Hit
GGCCAGGGCCAAGGTATGGCACAGATAAAGGCTGCACAAGCTCAGATTTC11.25No Hit
ATTCACAAGTCGATCATAAGGGGAACAGTGACGCGGCGTTCCGGCTGCGT11.25No Hit
AATCGGTAGGGCGGGGGGAAGCCCACCTTGGCCGGGCTGGCCACTGGCAC11.25No Hit
GTGTTGGCCCAAGTAAACTCTTGGGAGGGTTGTTAATTGCAGGTCGGAGA11.25No Hit
CCCCCACGCAGGTTCGCTTTTTAAAAGGGCTGACGGTCCCACCGTGGGTT11.25No Hit
GCTCTTGACTTCTCTACCTTCACCTGCGTCCTTCCCTTGCCCTCACCTTC11.25No Hit
GGGTGAAAGCATCCTCCTTCCGTTTTCCTGCTTCACTGAGTACAGAAGCA11.25No Hit
GTGCAGCCATGGATGACTTCCAACTGATTCCAATGATAAGCAAATGCAGA11.25No Hit
ATACGGGGCTCGATACCAGAACCCTGGGGTCATGACCCGAGCCGAAGGCA11.25No Hit
ATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCAT11.25No Hit
GATCCATATTTATAAGTTATCTAGGAAAAGGGAAAATGGAGTGGATAGTG11.25No Hit
GAAGTGACTGAGCCTCCAGAAAATATTTCCAGTGAATTAATCTCAGTGTC11.25No Hit
GCAAAAGGAATATACTCCTCTCAAGTGCCCCTAGAACGTTCTCCAACTTA11.25No Hit
AAATAATAAAGAGCTTTTCAAATCAAAAACACTGTAGCATAAATCCTGAA11.25No Hit
GGTTTAGTGTTAGGGTGAGGATTTAGAGTGAAGGACAAGGGAACAGAGTT11.25No Hit
AGTTAACACAAATGAAGCTGTCTTTATACCTACTGGAAGTACTCAGTAGT11.25No Hit
CTGTAACTCCAATGCTCATGTCAGCCGATTCATTAATTCCGGAAACTCCA11.25No Hit
ACATTTATGAGCTCATAGAAAACCTACTGGTTTGGAAGAGAAGGTAAAAA11.25No Hit
CTGTAAGCGCACCTGCGTCCTTCCCAAGTATTGTAAGCCTCTTAGTTGTC11.25No Hit
GAACTCTACTGGGATCGGAAGTATTTCCATAACAAGCCTGTTCGTGCACC11.25No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG11.25No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT11.25No Hit
GTTTATAGGGATGCCAGGGTGGCTCAGTCAGTTAAATGTCTGCCTTCAAC11.25No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTG50.0145.08
CCACCAG50.0145.02
AGGCCGT50.0145.07
CACCAGG50.0145.03
GCCACCA50.0145.01
GCCGTGA50.0145.09
CAGGCCG50.0145.06
TCATTCA50.0145.0145
CCAGGCC50.0145.05
ACCAGGC50.0145.04