FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1985_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGAGAAGTGTGAGGTATTAAAGTTACCTATGGTATTGGTGCTGATTTC15.0No Hit
GGATAGGAGACAGAGGAAAACCAGCTAGAAATGGGAGTGTGGGAAGAAGA15.0No Hit
GTGGGATTAGATACCCTGTGGGATTGTGGGATTAGAGATTCCACCCAGAT15.0No Hit
TTTCTGGAGTACCTGCACCAGTTTGCTTTCCCACCAGCGCTGTGCATGAG15.0No Hit
ATCCAAAAGAAAAGGGTACATTTCCAGAAACATACAACCTACTAAGATTG15.0No Hit
GCGGCTGTCTCCGCACAGAGGCGGGGCAGGGGCCACGGGGCGTCTCTGGC15.0No Hit
GGATTGGTATTCATTTTTTTATGTGGATGATTCGTAGAATTCACCGGTGA15.0No Hit
CTCCAGGGCAGTGGGCTGCACCTTCCCCAGACATGCTCCTCGGGAGTCCA15.0No Hit
NTTGGTAGCAGCGCTATCAAATGCTTTTTCTGCATATGTTGAGATGGTCC15.0No Hit
CTGTTCTGTGGCTGCCTTATTTCACAGAGCATCGTGACCTCAAGGTTCAC15.0No Hit
GACCATCGTCGATCCTTGGAAGGATGCAGCCTTCCTACGGTCCTACTTTG15.0No Hit
CAAATATCTACTCTTGGAGAACTCTTCTTCCGCAGGTCCCTGCCTGCCAA15.0No Hit
GCTCCAAGTTGTCCTGATTTGCTTTTGACACTGAGTTTCTGACATGGGTA15.0No Hit
GATGTTGATCCTTTGTTTTGTGAATGCGGCACATTAGTTTGATTTGTGGA15.0No Hit
GCAGGAGAGAGCCCTGGCAAGGGGAACAGCTGAGCTAGCAAGGGCGCGCG15.0No Hit
TCCTGGGTTCGTCCCGCCACGCCAAAATAAGCAGACACAGCCTCGACCCT15.0No Hit
TGTAATGCTCTTCATTGGCTCTTGTTACAGTGTTTGGATTTTTTCTTGTT15.0No Hit
ATGCCGGGTGTTGCTGGAAGGTCGAGGGAGTTGCAGAGAGAAGGACAATT15.0No Hit
TGTTAGTCCCCTTGCATAGAGAGAGCACTCCAGAGAGACTAGGCTCTAGT15.0No Hit
CCTCTAAGAACACCTCTAAGCACCAGCCTGGCTGTTTGGGCGAAGGAGCA15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)