FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1985_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAAGAGGGGTAGATCCTTTTTCTATGTCCCAGGCTTTTGTTCCT10.6060606060606061No Hit
GTAAAACGACTGTGGCCACACTACTTTGTCTAAGTCCGGGGTCCCCAGAA10.6060606060606061No Hit
TCTATGGACTGAGCCAACCAGGTGCCCCTTTAGCTCATTGTTACTACAGA10.6060606060606061No Hit
AGGAGAGATAGGTAGGATTAAAATAGAGGAATAGGTACTCCTGAGACTAA10.6060606060606061No Hit
ACATTCTACTGAACAACCAGTTGGGTTCATGGGCAAACCAAAATAGGAAC10.6060606060606061No Hit
GTAGTGTAAGGTTAGGGCAGACAAGTTTCGTTTGTTCATTTGGTTTTAAA10.6060606060606061No Hit
GGGGTGGCAGGTCCCCCTCCCCTCCTTGCAGCGTGTGGACGTCTCCTGTG10.6060606060606061No Hit
CCATAATACTTGACCGGCTCCCTGTAAATTAGTCTCCCAGGTGCCATTCC10.6060606060606061No Hit
GAGAGAGAGAACAAGCAGGCAGAGAAGCAGGCAGAGGGAGTAGGAGAAGC10.6060606060606061No Hit
CCCCAAGAATGGCCTTCTTATAACCCTGCTGCCCTTGGTGCCTGGCTGGG10.6060606060606061No Hit
TCCCAGAGGGGATGCCCTAGCAGAGAGGCTTTCGCTCTATGCGGGTTTTT10.6060606060606061No Hit
GTGTAGGAGTGGTTCTTGCTAATTTAATCCAAATTGTTCAAATTCTTGTG10.6060606060606061No Hit
CCTCAGCACTATTCGTAAGCTTCTGAAATCTCCATGACATGTATATTGAA10.6060606060606061No Hit
CCTCGGTGATCCCTCAAATTAGGGCCAGAGACAGTTGATGATTTTATACG10.6060606060606061No Hit
CTCCAAGCTTCCACATCCTGGAGGAAGACGGCATCTGACTCCCCGTTATT10.6060606060606061No Hit
GAGAAGGACAGCCTCGGCCAGCAGATCCTTCCAGCACTCCCTTACCTGCC10.6060606060606061No Hit
GTAGTCAATTAGGGGCCCTAGCCATTCCTTTTTTTTTTTTTTAAAGATTT10.6060606060606061No Hit
GTCCATTTGGCCCCCGCATCTATGACTGGATAACTCTGTTGCCTGATTCT10.6060606060606061No Hit
GGGCGGGCTTTTGGCGGGCAGCTGGGCTCTCCGCTCGTCGCGCGTGACTG10.6060606060606061No Hit
GGGCACGCCCCCTCTGACCATCAGCAGCAGCCAGCTTGCTTTAGTTCCCA10.6060606060606061No Hit
GTCCTTCTATCCTCTGTCCCTCATGTCCTGAGGTCAACTGCACCACATCA10.6060606060606061No Hit
TCAAATACCCATCGGGCTCCATGTTGAGCAGGAGACTGCGACCCCCTCTC10.6060606060606061No Hit
GTCTAGGGAAGGCCTCCCTCCGTGCCCAGCCTTCTGACCGCATCGCTGAG10.6060606060606061No Hit
GAGGTAAGTTCCAAAAAAATACAAATATATAATGATGATGTCTTCTTTGG10.6060606060606061No Hit
CCCCTAAAATGCATGAAGCACTCACTAAGGTCAGACTTTAAACTGAAAGG10.6060606060606061No Hit
AAGCAGTAGAGTCAGCAGGAAGCCTTGATCTGGTTGTCTTTGGTATCGTT10.6060606060606061No Hit
GCCACCAGGGTGGCCCTGCAAGATCCTTTTTATAGCCAACCCCGTAGAAA10.6060606060606061No Hit
GACCTCAACTGACGGGCCTCAAGTCTCAGCGATGCGGTCAGAAGGCTGGG10.6060606060606061No Hit
GGTATAATTTCATTTGTTTTTTTCTTTTTTTAAAATGACTTATTTATTTA10.6060606060606061No Hit
GGGTATGTGTGTGTGTCCCAGTATGTATTCTTGCACATGTGTGTGCGTGC10.6060606060606061No Hit
GGTCCGTACCCTGCACCTGGCCAACACCTGTGGGAGGCCGGACACCTAGC10.6060606060606061No Hit
GTTTAAGATAGCAGCTAAGGTTGTGAAGCAAAGGAAAGAGTCACAGGAGA10.6060606060606061No Hit
CCTCTGGGCATCTGGAGGCTGAAGGAAGAGCATTCCAGGTGGAAAGAACA10.6060606060606061No Hit
ACCAATTGGGTTCATTGGCAAACCAAAATAGGAACACAGAAATACTTTGA10.6060606060606061No Hit
TATTCACACCCTCCTGAAACACATCTCTAGAACATTTTCCCCTTTCATTG10.6060606060606061No Hit
GCCTGAGTTTGGCTGTCAGTTTTCCGGGGCTGCTTCAGGGCCCCAGTGAG10.6060606060606061No Hit
GCTCATATCGTGATCCCAGGACCCTGGGATCACGATATGAGCTGAAGGCA10.6060606060606061No Hit
ATATACATGTGTCAAAACTCTTCAAATTGTGTTTGCTTGAAAAAATTTTC10.6060606060606061No Hit
GTTTACACATTCTCATGCTGGGCTGGAGGGCAGAAACGTAAGGCCCGTCA10.6060606060606061No Hit
TACCAAAACCATCAGCTTCTTTTAGAGCACGAGGGAGAACTAGGCCTGTT10.6060606060606061No Hit
CACCAGCTGATGGTGGCAGGTGTCTCAGGTCGAGTTCACCATTATCCTAC10.6060606060606061No Hit
GGTGGGGTGACTGCTCTCTGTTGGGACCCTGTCCAGCGGGTGTTGTTCTC10.6060606060606061No Hit
CCCCATTTTAGAACCTGTTTTACTGCATTCTGTGAATTTTGTTTCATTGT10.6060606060606061No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG10.6060606060606061No Hit
GAATAAAGTCCACATTGTTTACCATATCCTGCAGTGGTCTCTATATCTAC10.6060606060606061No Hit
GCCCAACTTCAAAGCCCACAGTTTCTCCCTCTCCGAACTGTGTCTTCAGT10.6060606060606061No Hit
GAATCATACTTTACTGTCAAATCTTTGCAAACGTTGCTCTTCTCCAAAAT10.6060606060606061No Hit
AGCACAGGCTCTCTGAGCAGAAAGCCCGACATGGGGCTTGATCCCAGGAC10.6060606060606061No Hit
GCTGAGCGGTGAGCCCGATGTGGGGCCTCGGTCCCAGGACCCTGGGAACA10.6060606060606061No Hit
GGATACATTTCCAGAAACATACAACCTACTAAGATTGAATTCTAAAGAAA10.6060606060606061No Hit
AGGTTATACCGTTTGGGCTGGGGACACTCTCCTTTCGTAAGGAAACTGCC10.6060606060606061No Hit
CTTGTAGGACTGAAAATACTTGATGCTAGAATTCCACATCCAGCTAGGAA10.6060606060606061No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10.6060606060606061No Hit
GGCCTTAGCGCAGTATTTGTAATGTAGGTGCTCGTCATTTTTTGAGTAGC10.6060606060606061No Hit
GTGTAACTGTGAGGAAACCGAAGAGTCCCGGAATATTATACATTATTTAT10.6060606060606061No Hit
GTTCTTAGCTCCTACCTGGTGCCAAGTCCCAGAGCGGCTTCCCCATGAGA10.6060606060606061No Hit
CTCCTGAGCTCCTGATCAGTTGCCTTGGCTGTTTCTACCCTACAGCCACA10.6060606060606061No Hit
GTCCCAATAGGGCCCCCAAAATGTCCACTTGTTCTTGAACCAAATCCACT10.6060606060606061No Hit
GGGATAGAGCCCTCCATTGGGTTCTACACTCAGTGGGAAGTCTGCTTCTC10.6060606060606061No Hit
GCCCTGCAGACATCTCGGACCTCGGCTCGGAGCTGTCCCAGGCGCCGCCC10.6060606060606061No Hit
ATTATACAGAGCTGCTCTGAAGACTAATCCAGTTATGAAATACAAGATGC10.6060606060606061No Hit
CCATTGTTCTCTTCTTCCCTTTAAAAAAATAATTCCAAAAAATCCAATGG10.6060606060606061No Hit
GGGTAACTCAGTTGGTTAAGCATCTGCCTTCAGTTCAGGTCATAATCCCA10.6060606060606061No Hit
GTCCCCCCCCTGCTCCCCGTCACAGCTCAGTTCTAAGGCAGGGCGCTTCC10.6060606060606061No Hit
GTTGTAGTGCCCTTCATTGGCTCTTGTTACAGTGTTTGGATTTTTTCTTG10.6060606060606061No Hit
CTTTTATACATATTCTCGTATTGGGAGTCAGGATATGGTCATTGGAGAAG10.6060606060606061No Hit
GATCAAGCCCTGCATCAGGCTCTCTGCTCAGTGGGGAGGCTGCTTCCCCC10.6060606060606061No Hit
CCCTTGGTGTACAAGACTTCCTCTGTTGGTAAAAATGAGTTTTGGGGTGG10.6060606060606061No Hit
GAGTACACAAGCCTAGAACAACACACACACACACACACACACACACACAC10.6060606060606061No Hit
GGTAGAGATAGAGACCACTGCAGGATATGGTAAACAATGTGGACTTTATT10.6060606060606061No Hit
CCAGTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAG10.6060606060606061No Hit
GTCCATCCCTTGTGCAGAACACAGGGGAATCAGGGGGTCGTCAGTTTCTG10.6060606060606061No Hit
AGGAATAACTAAGGCTCTTCCTTTCCAAGAAGCTATACTGGTAAAAATTA10.6060606060606061No Hit
ACACCAGTTAGAATGGCCAAAATTAGCAAGACAGGAAACATGTGTTGGAG10.6060606060606061No Hit
CTCTTAACAGCCTTTTGGTGTGCTGAAAGAACACGGAACTGAAAGAGTCA10.6060606060606061No Hit
AGACTAGTCCTGATACGCAAACCAAGGATCTGTTTTAAAGAGACAGGTGT10.6060606060606061No Hit
GGGTGCCCCCCACTGTGTTTAAAGGACAACGGATTTATATTCACGCTCAT10.6060606060606061No Hit
ATGCAATATGTGCCCTCCATAGTACCCATCTCACCCAACCTCCTACCCCT10.6060606060606061No Hit
CAATTATTGAACCTTATCATAGTCATGTTTCATTTCCTTACAATAATTTT10.6060606060606061No Hit
ATCCCAGACTGATTCATGCTGTGGGGGCCAAGCCTGCTTCCAAACTGGAA10.6060606060606061No Hit
CTTTCTGTCTCTACAATAAATACATATGTCTTTAAAAATCTTATCCTGAA10.6060606060606061No Hit
CAGTGGAATCAGGGAAAGCCTGGTTAAACTGGGAGGTGTGTGTAAGCTGA10.6060606060606061No Hit
GGGACAATAGGTACTGAGCTCTTAGAGCAGCCGAGGAGTTCCCAGGAATA10.6060606060606061No Hit
GAGTGGGAGGTGGAGAAGCAAGCTTCCCATGGAGCAGGGACACCAATGTG10.6060606060606061No Hit
GGGTGTGTGTAGGGCAGGAAAAGATAGGCAGGCTGTCAGGCTTGAACACG10.6060606060606061No Hit
TTCCACAACTGCCCAATGTGTTACAGAGTATGAGCGTGAATTTAAATCCT10.6060606060606061No Hit
TTGCCACCCTGACCAGCAGTAGGGAAATCTGGGTGCCATGGAGAAATGGC10.6060606060606061No Hit
GTGCAGACCGCAGGTTCCTCGACACCCACAGCCCAGGCCGCAGGGAGACC10.6060606060606061No Hit
CCGCCAGGCGGCAGAAGCACAGGTCGGGGTGGGGCAGTGATTTGGTCGCA10.6060606060606061No Hit
CTTCCCCACAGGGGGGCCTTGGTCAAGGGTGGAGCTCCCTTCTGGTTTCA10.6060606060606061No Hit
GAGCACTGGTTGTGGTGCAAAAATAATGAATACTGTTATGATGAAAATAA10.6060606060606061No Hit
GCCACCCAGGCACCCTTTTTAATCTTATTTTAGAGACATTTTAGGTACAG10.6060606060606061No Hit
GTGCATTTTCATCACAAATAAATTTCACAGTACTTCTTGGTCTTCAGAAA10.6060606060606061No Hit
CAGGATATCCTCATGGGAGAAGAAATTATTGTGTTAATTTACAGAGGAAA10.6060606060606061No Hit
CGGTTTCTCTCTGAGAGAATAGTGTCAGCAAGTTTTCTGAAAACGATTGT10.6060606060606061No Hit
GTAGAGTCCCAGTTCTTGAAGGATGGCAGAGGACAATTTGTTGCCAAAAT10.6060606060606061No Hit
GGTCTAACCAGGGCCCAGTGCCTGGCACATGGCACCTGGCCGTTTCTCTG10.6060606060606061No Hit
ATGGAATCAGGTGTGTATTCAGGCATGTATAAGATGGGTCAGGAGAGGAG10.6060606060606061No Hit
GAACTTACCTTGTCAAATCATTTGTTGGCTCAATTTATACTTTCCTAGTG10.6060606060606061No Hit
CATTAACTGTTGTCTCAAAGGTACTAGAGTCATTTTGTTTATAAGAACAG10.6060606060606061No Hit
GTGTCATTCAAACTACCAGAAGCTGAGAAGGACAAGAAAGGATTCTTCTG10.6060606060606061No Hit
GGGCAGAGGGAAGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGG10.6060606060606061No Hit
GTATAATGTTAGTAGATACGATATCGATATGAATATAGCTTTCCTCTTAT10.6060606060606061No Hit
GAGTAGGGACTCCAACGTGGGGCTCGATCCCAGGACCCTGGGATTATGAC10.6060606060606061No Hit
GTTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAG10.6060606060606061No Hit
GTTTCCACCCTGGGACCGATCAGAGGGTCTCTATGTCTAACATAGCGTGT10.6060606060606061No Hit
GGTGGGGGGGGGCAGTGATTTGGTAGCAGCGCTATCAAATGCTTTTTCTG10.6060606060606061No Hit
CCTCACGCGTGCCCTCTCTCACCTGATGGGAGAGCAGCCATGGGCTTCAG10.6060606060606061No Hit
GGCTAGACCCTTCTAGGTGAGTAGTCCGCTGGCTCTCCTGAGAATCTTCT10.6060606060606061No Hit
CTTGAGTGAGGACTCATAGAAGGTGAGAAAGGTAAGCAGATTTCAGAGGG10.6060606060606061No Hit
CTTCTTCAGAGTCTCGAGTGGGGCAATCAAGACAAAGGACAAGAATATGG10.6060606060606061No Hit
TCCAGGCACAAAGCAATGGCTTCTTGCACATGCAGACCAGCACAGATAAC10.6060606060606061No Hit
GCCTTGGCTGTTTCTACCCTACAGCCACAGAGCCTGACTCCATCCAACCA10.6060606060606061No Hit
GGGGTATATGTAATGTAAATCAATTGATGGATGTTTTAAAAAGTAAACTC10.6060606060606061No Hit
GGGCTTTCTCATTTCTCTGTTGGGAAAGAGCACAGCCGGTTTTCAGGAGC10.6060606060606061No Hit
GGGAAGTGTGAGGTATTAAAGTTCCCTATGATATTGGTGCTGATTGCTTC10.6060606060606061No Hit
CTCAAGGGAGGCGGGATCAAGGAGTTGGAACAGAATCTCTAGGTCCAGGT10.6060606060606061No Hit
CACACATCCACACACACACCCCCCCACACACAAAATAGCACATTCACACA10.6060606060606061No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT10.6060606060606061No Hit
GCTCTCTACTGCTCATTGGTCTGTTCAGATTTCTGTTTCTTTAGGATTCA10.6060606060606061No Hit
AAGTAGACCAAGTGACTTCCAGGATGCCAGTCCATGAGTGGTAAAGATGA10.6060606060606061No Hit
GTGGTGTCCCCAGCATGCCACAGGGAGCCAGTAGTGGAACTGTTTGTGTC10.6060606060606061No Hit
CATTTGTGGACGTGCACAAAGTGGTGAGAAATTTCATTATGGAGCACAAC10.6060606060606061No Hit
ATTGGGCAGTATCTCCTACTAGTTTATAAAGCACTTTTATACACATTCTC10.6060606060606061No Hit
GGGTTATTCATTCATTTATTATGTAGTCAGCAATATATTTATATAAACAA10.6060606060606061No Hit
AGCCAAAACCAAGCATTGGCAGGGATGGAGGTCACAACTGGGAAAGTTCT10.6060606060606061No Hit
GTACAGCACATAGGGGCGTATTCGGATGTGTTTCACAGGCCTCCTTTATT10.6060606060606061No Hit
CATGCGACCTGCCCACAACAAAACTCGGAGACCAGCCCTTCTCCCTGTAG10.6060606060606061No Hit
GGCAGCTGCTGTCAGGAGAAAGTGCCAGCAGGTGAAGAGGCCTCGGATGT10.6060606060606061No Hit
CATTTGGAGGAGACCCTATGTAGACAATTTCTCTAAGGAGTCGTCTGGCC10.6060606060606061No Hit
GTATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10.6060606060606061No Hit
GTCCCCGAGTCCGCCTGAGGGCCCGAGCACAAGGCCAAGCGTTGCGTACA10.6060606060606061No Hit
GTGCTGTTGATTAGGTTACATTTGGAGTTAATTTGTTCGTTGGTGGTTTC10.6060606060606061No Hit
AGTTAAGCTCCAATGCTTTGACACCCGCTCCTTCCCACCCATATGTTCCA10.6060606060606061No Hit
CTGTTCCTTCTGCAAGGGTGTTCACAGCTTCCGCAGTGGGTATAGTTGTA10.6060606060606061No Hit
ATGTAGGGTCGGTGGCCGGTCCTGAGACAGATCCCCGAGCAAAAAGCCTG10.6060606060606061No Hit
GGGGGGGGGGGGGGGGGGTCTAGGGGCAGTTGAGAGGAGTAGATTCGCTT10.6060606060606061No Hit
GTTCAGAGTGAAGGCTGAATGCGAGAACAGACTTCCCACCTCCAGTCTTG10.6060606060606061No Hit
GGCACACCTATATTTAACTTCTGACAGTGTAATTATAATGTTTTTCATTA10.6060606060606061No Hit
CTATAACTTATGCTTGGATGATTTTCTACCACATGTGTTTTCTTTGTAAG10.6060606060606061No Hit
GGCCACTGCTACTCAGAAGCCAAGAAACTGGACAGCTGCAAATTCCTCAT10.6060606060606061No Hit
CCTGGGAGAGAGCCCCGCACCAGGCTCCACACTCAGCAGGGATGCTGCTG10.6060606060606061No Hit
TCGTGAAGCATGGCAAATTATTTTGGTAAGACACAAAATCTTTGCTGTTC10.6060606060606061No Hit
GGCCAAGAGAGAGAAAAGCAGCCCTGTCATCCAGGCAGTGGTCTAAAGCT10.6060606060606061No Hit
GACGTGTAGCGCTTGGGGCTGAGATCCACTCCACCCAAACCTGATGGATT10.6060606060606061No Hit
GGGTGGGGGTGTTCTAGGGCAGGACAGTGGGAGATGCTGGGTGCTAATCT10.6060606060606061No Hit
TGGCAACTGGGGGAGGACGTGGCGTGTTTAAGCTCCAGTCTTGTAAGTCA10.6060606060606061No Hit
ATGTATTCATCTCTATTCCCATGTCAGGGGGTCGGTGAGCATGACCCAGG10.6060606060606061No Hit
GGACCCCAGGAGGCCCCAATTGCCCTCTAATTTCTGGATCCTGGGAGCTC10.6060606060606061No Hit
CTGTTAGTGTATAAGAAAGCAACTGATTTCTGTACATTGATTTTGTATCC10.6060606060606061No Hit
GGAGGCAGAAGCGCAGCTTTCACACCATCCTGCTGGCCCTTCTCAGCCAT10.6060606060606061No Hit
GACATACTCTCATTGTGAAAGTTGAAACCATAAGGCTTCTTAAAGAAAAT10.6060606060606061No Hit
GGGCCGGTCCCTGTGACCCTGTCGAGCTCGGCTCGGATGGTGTGCCGGCT10.6060606060606061No Hit
CTGCAGGATTCGGACGTGATCTGTGTAAGTGGGAACGTGCAGGCTCTGGA10.6060606060606061No Hit
GAAGTTTATAGTGATACAGGCCTAACACAAGAAACAAGAAACATCTCAAA10.6060606060606061No Hit
AGTTAAGGCAGAAGAAGGGAGTACTCCAGCTCTGTTTCCATGAGGCCGCC10.6060606060606061No Hit
CGCCTGGGGGGCTCAGTGGGTTAAAACCTCTGCCTTCGGCTCAGGTCATG10.6060606060606061No Hit
GCTGTGAAGTGTGTAAACCTGGCGATTCACAGACCTGTACCCCTGGGGCT10.6060606060606061No Hit
CATTAAGCTCGCCCCCGAGGTGAGCCCGCCAGTGGCATCGCTGGCCCCAG10.6060606060606061No Hit
ACCCACCGCTGTCACTTCCACCCCCCCATTCCTGACATGCTGCTGCCACC10.6060606060606061No Hit
GTGTGAGGTATTAAAGTTCCCTATGGTATTGGTGCTGATTGCTTCTCTTA10.6060606060606061No Hit
CAGCATCCCTCTACTAAGTAGACCAAGTGACTTCCAGGATGCCAGTCCAT10.6060606060606061No Hit
CCCTGACACTAATGATAATAATAATGATGATGATGAAAGTAATAATAGTA10.6060606060606061No Hit
GATTTGCTCTGTATTACTAAATTGTATCAATTGTTACTAAAGTGACTGAT10.6060606060606061No Hit
TGGTTAACCAACTAGTCCCCTCGGTGTCCCCACGGGAGAAACTTTCTGTT10.6060606060606061No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers