FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1985-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105202
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11430.10342000828807765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG5650.041.067215
GTGATCG1701.8299033E-938.3820659
TATACGG1102.4344811E-432.958775
GGGTCGA3300.032.9542969
AGCGAAA4650.029.6355021
GCGAAAG4900.028.115852
GTCGTAA1306.4682454E-427.8957651
TATACTA1409.987782E-425.896185
CGAAAGC5450.025.2784733
GTATAGG3352.1009328E-923.815491
GTTATAC2852.7759052E-722.8976733
TAGTACT3203.381865E-822.6591574
TATAAGG6550.022.1402443
ATATTAG8200.021.2279971
CGCTTAC2053.6568908E-421.2279971
TTAGAGT6500.021.1950264
ATTATAC4451.1277734E-1021.1824913
TTATACT6050.020.3745124
GTATTAT8900.020.3733121
TACACCG2155.052227E-420.2351555
Produced by FastQC (version 0.11.5)