FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1985-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697255
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3950.033.042473
AGCGAAA3550.032.6875341
GTACCGC950.00472318230.5304666
GTGATCG1703.0101655E-629.852729
GCGAAAG3900.029.7476392
GTATAGT2104.967533E-727.628751
ATCTAAA6500.026.7786331
AAGTCCG1658.407203E-526.3672225
AACCGAT1409.991981E-425.8926667
TAATATG3052.0294465E-823.7737244
ATAGTTC1902.190226E-422.897853
GTTATAC1600.00217277622.6593323
GTAGGCT3004.5448905E-721.7529586
GTCTACA2104.3021192E-420.7215611
GCCTATT1750.003650198520.7215611
TATGGGG2455.105803E-520.7171043
GAGTGAT3851.0499207E-820.7141327
CGTAGAT350.00366068720.714132125-129
TATACTG3208.452098E-720.3933985
GGCTCCG3208.4637395E-720.3904749
Produced by FastQC (version 0.11.5)