FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1985-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396218
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC24680.17676322751891183No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15980.11445204115689671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1951.70985E-1037.179459
TATACTG5700.035.6145
TACGTTA1204.0588863E-430.2083035
GTATAAG4200.029.3483621
GCTACTA5100.025.588219
TAGTCGG1450.00122669424.9999758
GTATAGG3902.0008883E-1124.169241
ACGTTAA1801.5207163E-424.1666436
GTATTAG4600.023.643821
ACCGCTC1902.1937877E-422.8947148
GTTGACA14000.022.7881411
TTATACT5550.022.2071864
GTATTAT10450.022.203321
TACACCG4256.184564E-1122.1764495
ATACTGT9400.021.5957246
GTATTAA10650.021.1055341
TAACGAC1750.003659466520.7142663
GTATATA6400.020.3927961
TATAAGA5353.6379788E-1220.327812
GAATAAC8600.020.234711
Produced by FastQC (version 0.11.5)