FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1985-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925092
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10670.11533987970926134No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9950.1075568700194143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2350.058.62951
TTACGTG3300.039.5474622
TACGTGT3500.035.2160723
GTAGTAC1301.6540733E-533.4632383
CTAGTAC1956.9867383E-933.4632343
CGTGTTG4150.031.4473785
ACGTGTT4350.028.334774
GTACTAG1804.691685E-628.2004471
GTTATGT6750.027.9318711
TACTGAA11750.026.5318267
TTTACGG1100.00966882926.3649713
CTACTGA12550.024.2641776
GTAGCAT15850.023.7905521
TATACTG4303.6379788E-1223.6058465
GCATGTT11800.023.348644
TAGTACT2802.3410757E-723.3047524
TAGCATG12600.021.8661862
CATTCTA8300.021.8384572
TTATACT4801.4551915E-1121.1469064
GTTGGCC15850.021.040948
Produced by FastQC (version 0.11.5)