FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1985-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1985-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755254
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC10200.1350539023957503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG502.0073162E-458.0152551
TAGACCG450.00963829948.333255
TACACCG1751.8189894E-1245.5713465
ATCGTCA800.002036318236.2499358
GTTACGT1301.651272E-533.470341
AGCGAAA2755.456968E-1231.6446861
GCGAAAG2807.2759576E-1231.0713732
CCGTATA1652.3805187E-630.7656671
CGTATAC1453.4893194E-529.9999472
ACACCGT4400.029.6590376
CCGATCA1000.00607358328.9999479
ACCGTAT1255.152715E-428.9999478
CGAAAGC3052.3646862E-1128.5245383
GACCGAT1306.4815395E-427.8845637
ACCGATC1306.4815395E-427.8845638
ACCGTTC2001.0650338E-525.3749548
TTACGTG1450.001226178424.9999542
CACCGTT1450.001226178424.9999547
GTGTAGC2951.4228135E-824.5827371
CACCGTG5100.024.1666227
Produced by FastQC (version 0.11.5)