FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1984_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1984_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCACCTTTCCAATAGA17.6923076923076925No Hit
ATGATGGATCAGGTGCGAGAAAGCAGAAATCCTGGGAATGCTGAAATTGA17.6923076923076925No Hit
TTATAGGGGCAATGCTCAAGACATTTCTAAACCATTCAGGTTGGTTCCTT17.6923076923076925No Hit
ACTCAAAAGAGCGGTTTTTACCCTGTTCAAGACTCCCAATACAAAAATAA17.6923076923076925No Hit
ACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTC17.6923076923076925No Hit
TTGTAAGCCTCTTAGTTGTCTTGTTGTAGTTAAATGCAGGGGAGTTACCT17.6923076923076925No Hit
CATATAGTGGAGCGGATTCTGGAGGAAGAAACTGACGAGGAACTTAAAAT17.6923076923076925No Hit
AACTCACTCTGGATAAAGCTTGCTATCGATCTGAGCTCTGGCTCATCACT17.6923076923076925No Hit
AACGAGGCGAATCAATAATTGTGCAATCTGGTGATCCAAATGCATTGTTG17.6923076923076925No Hit
ATTTTCTTCTCTGACTGTGAATGTGAGGGGATCAGGAATGAGAATACTTG17.6923076923076925No Hit
TTCCAGAAGTTTGCTTGAAGTGGGAGTTGATGGATGAAGATTACCAGGGA17.6923076923076925No Hit
CAACTAAGTATTTGAGGCATGTCCCTAAATCATTTGGGAATATCTGGAGG17.6923076923076925No Hit
CCACAATATCAGTAAATATGCATTTGGAACCTGCCCCAAATATGTAAGAG17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)