FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1984-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1984-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732333
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG350.003566249362.14239
GTACTAG800.002033620436.259581
GTGATCG1658.416065E-526.36349
ATATAGG1450.001224242625.0066051
GTATTAT6150.024.7626381
GTTGACA6200.024.562941
GCACCGT5050.024.4107236
ATTATAC2101.5519732E-524.1714023
GTTATAC1500.001492188124.1714023
TATACTG2903.2773642E-722.5044085
GTTGGAC3004.5429078E-721.7542633
GCGAAAG3055.322581E-721.3990961
GTTATTA2053.6586108E-421.225121
ATATGGC1750.00365365720.7183463
TAGTACT2104.3069143E-420.7183444
TAGGTAG3951.4071702E-820.1938295
TATCAAC1800.004301106620.1428363
ACATGGT6950.019.8240285
AGCACCG5153.8198777E-1119.7125995
TTAGAGT4452.5956979E-919.554394
Produced by FastQC (version 0.11.5)