FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1984-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1984-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538744
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG5930.11007083141529186No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC5570.10338862242549338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA450.0096367948.333276
AGGTACT3700.045.0675134
AGCGAAA1652.7284841E-1143.9474951
GGTACTG3500.043.4999435
GGTCGTT1002.7318056E-643.4999437
GTATTAG1053.815985E-641.436211
TATTAGG800.00203579536.2499542
TACTACA1604.5829438E-836.2499544
GGATAGC2501.8189894E-1234.7999535
TATAAGC1051.8525978E-434.5237662
GTACTGA4900.034.030576
ACTGATC5000.033.3499578
GCGAAAG2206.202754E-1032.9606251
TATACTG3100.032.7418945
CGAAAGC2455.4569682E-1132.550983
CAGGTAC4600.031.5216983
GTACAAT950.004725409730.526286
GGTTATA1453.483049E-530.0055331
GTATGGA3450.029.4257141
TTATACT3001.8189894E-1128.9999644
Produced by FastQC (version 0.11.5)