FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1984-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1984-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194959
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC3460.17747321231643576No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC2270.11643473756020498No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG100.0070865527144.999745
TATATAG502.0062318E-457.9998933
GTATATA400.006043652554.3749052
GCTATAA604.9290276E-448.3456461
TATAGTG752.542995E-548.333255
ATATAGT700.001054556341.4284974
ACGCTAA1001.3852664E-436.259231
TACAAAC1251.2598963E-534.799943
GATTAGT950.00471810630.526262
CCGACTG1000.006062683628.9999479
GAGTATA2303.457535E-828.369513145
TATAAAG1050.00769355527.6193
ACATAGC1100.00964088426.3703541
CGCTAAG1409.965777E-425.892812
TTAACCA1500.001489986224.1666244
GTGCAAG2151.8557903E-523.604616
ATGTACT1851.8233388E-423.5134744
GACTTGG1550.001800622723.3930551
TCTATAC2852.755114E-722.8946973
TAGCACC1650.00259345321.9696584
Produced by FastQC (version 0.11.5)