FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1983-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1983-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634880
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6410.10096396169354839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG259.382425E-487.0063864
GCGAAAG2154.8567017E-1033.7234042
TATACTG2351.2605597E-930.8533255
GTCCTAT6050.029.9703181
GTACGTG1000.006072970628.9998449
GTATTAT4600.028.3805851
TACGGGG1306.480527E-427.8844647
TATATAC3950.027.5336653
TATTATA2156.114751E-726.9787222
GTACCGG1907.145978E-626.7124846
CGAAAGC3005.857146E-1026.5852813
ATACTGT5100.025.5901136
CCCTATA1701.030198E-425.590112
GTATATA4400.024.7255121
TAGTACC2351.3283116E-624.6826614
TACCTAT1801.5194883E-424.1665367
TCCTATG7600.023.8504312
ATACGGG2502.2755867E-623.2017026
GTCTTAT2003.0931775E-421.7584511
CTATGAT8050.021.6164934
Produced by FastQC (version 0.11.5)