FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1983-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1983-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494631
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG7630.15425640527989554No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT6540.13221977595419618No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6480.13100675048672647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT100.007087673145.014471
GTGATCG554.0586056E-665.9099
AGCGAAA1656.001028E-835.155021
TAGTACC1707.809285E-834.11769
TACTGCG1102.434398E-432.95455
GCGTTAA900.00362649732.222189
GATATGT2601.10958354E-1030.6761341
GCGAAAG1902.0711741E-730.5293582
GCATAGT950.004722706530.5293581
TATTGGG3151.8189894E-1229.923622
GTTGACA6000.029.0028921
CATAGTG1255.147116E-429.0028922
GTCTTAC1000.00606857329.0028921
TATATGG2502.4410838E-928.999963
GCTATAT1050.007700997427.6218011
GTCTAGC1050.007700997427.6218011
ATACACG1050.007704798627.619016
GTATTAT5800.027.502741
TATACAT2156.1141327E-726.9767075
GTATAAG1358.072205E-426.8545281
Produced by FastQC (version 0.11.5)