FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1983-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1983-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682255
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14860.21780712490197945No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT8740.12810459432323693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG700.001056425941.4284029
GTATAGT1501.1312786E-633.8406371
TATAATA3000.031.4188422
TGCGACA950.004724654430.5284272
TCGCCAT1255.1522657E-428.999884145
GTGTAGC2207.466024E-726.3693281
TATACTG2901.1883458E-825.0017325
TTGGACA4350.025.001734
TAGTATG4750.024.4227432
GTAGTAT4850.023.9226881
CAGTATG3352.1063897E-923.8058749
ATACTGT3402.4992914E-923.4575086
GTATAAG1550.001805634123.3921431
TAGCATG8100.023.2732162
GTAATAT1600.0021717222.6611421
TTTGGAC4800.022.6578183
TTAGAGT2252.64853E-522.5571164
TTATGCC2252.64853E-522.5571163
TAATAGT1650.002598975821.9712184
GACAGTA3355.4926204E-821.6417057
Produced by FastQC (version 0.11.5)