FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1983-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1983-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663925
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA21060.317204503520729No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT16640.2506307188311933No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15540.2340625823699966No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA13910.20951161652295064No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11680.17592348533343374No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG8760.13194261400007531No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT8140.12260420981285537No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA7810.11763376887449636No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG7710.11612757465075121No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG7570.114018902737508No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC7150.10769288699777836No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA7010.10558421508453517No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT6980.1051323568174116No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6700.10091501299092519No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6680.10061377414617614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCC100.0070904163144.99949
GTAGAAA6550.053.1335532
GGTCGTT3450.048.3331347
GTCGTTT3600.046.3192528
TATACTG8550.044.944725
AAGGTCG3750.044.469835
TTATACT7100.041.8691754
AGGTCGT4200.039.7052086
ACTCCGC951.02854254E-438.1577348
GTGATCG1354.9681876E-737.5924349
TAGAAAC9400.036.252583
ATACTGG9450.036.0607646
CCTTATA8800.035.4286582
AGCGAAA3200.033.9919131
ACAAGGC7350.033.5372778
GTATTAG1102.4321023E-432.9618571
GCGAAAG3750.032.8739551
GACAGTA3550.032.675927
TAACCAC3800.032.436525
CGAAAGC3600.032.2245182
Produced by FastQC (version 0.11.5)