FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1982_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4771
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA160.3353594634248585No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT120.2515195975686439No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA110.2305596311045902No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA110.2305596311045902No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA100.20959966464053656No Hit
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC100.20959966464053656No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT100.20959966464053656No Hit
TTATTATACATGCTGTATTCATGGAAAACCAAGACCTCTGAAGGGACTCG80.16767973171242925No Hit
GATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCT80.16767973171242925No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT80.16767973171242925No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG80.16767973171242925No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA80.16767973171242925No Hit
ATCATGGCCTGATCGGGGTCCGGTCTGAGAAGGAAGGGAGGAGCCTGGGG80.16767973171242925No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT80.16767973171242925No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT70.1467197652483756No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG70.1467197652483756No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG70.1467197652483756No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC70.1467197652483756No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT70.1467197652483756No Hit
GCTTAATGGACCACCCCTCTCGTCATGGCCCGAGAAGCTAGTTTGTCTTC70.1467197652483756No Hit
ATGTAGGACTGTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACAGA60.12575979878432195No Hit
ACTCAAAAGGCAATTGATAAAATAACATCCAAGGTGAATAATATAGTCGA60.12575979878432195No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA60.12575979878432195No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT60.12575979878432195No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT60.12575979878432195No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA60.12575979878432195No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA60.12575979878432195No Hit
TTAGTATACTGGCCAAACAAATGGACACCCAGATTTGCTAGAGCAGCCCA60.12575979878432195No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC60.12575979878432195No Hit
ACCTTGCACAGGTCACAACACTAAGCATGACGGATGACCCCTTCCCCTCT60.12575979878432195No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC60.12575979878432195No Hit
GAATGAATGAATGAATACATGAATGGAATTTCCTGAAAAAAGTCCTTTTA50.10479983232026828No Hit
GTTCTGGACCCTCCCTTCTCTCCTCCAGGCCCAGGTTCCGGCTCCCGCTT50.10479983232026828No Hit
GCTATAAATACATGCAGCGTGTTAGTTACACACCTTAGCTCAGGACGCAG50.10479983232026828No Hit
TCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATC50.10479983232026828No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG50.10479983232026828No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG50.10479983232026828No Hit
CCTCAAGACCATGAGAAGTCCCTGCTGGCTCCCTTCCTGAAGGTTCTTCT50.10479983232026828No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG50.10479983232026828No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG50.10479983232026828No Hit
GGAGAAGAATCCCGCTCTTAGAATGAAATGGATGATGGCGATGAAATACC50.10479983232026828No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT50.10479983232026828No Hit
GTAGAGTGCTGTGCATACTTAAAGCTATAAATACATGCAGCGTGTTAGTT50.10479983232026828No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT50.10479983232026828No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG50.10479983232026828No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.10479983232026828No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC50.10479983232026828No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA50.10479983232026828No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG50.10479983232026828No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG50.10479983232026828No Hit
GTATTAGCCAATAAGGATTAACAGCTCTCGGGGTGATGGCATATTAATGA50.10479983232026828No Hit
CCTCTCAATACTGCAGACTCTACTCCTGCTGTTCCTTCATCTGGGTCCTC50.10479983232026828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA100.006871769145.000029
CTCATAG100.006871769145.000021
Produced by FastQC (version 0.11.5)