FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1982_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATACTGTCTCTTTCTGGATGTTCAGACACAGTGATCTATCTGAGG50.17018379850238255No Hit
GGGTAGCAGTCTCCATGGAGCAGGGACTTAAGCCTCCTCCAGCTGATGAA40.13614703880190604No Hit
GAGCTGGACATGGGCAGTCATCCCGTATGGTTGGCTGTTAAAGCCATGAG40.13614703880190604No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC40.13614703880190604No Hit
CTCCTGCAGAGATCAGAGAGCCAGAGCTCACTCCGTCCTCTGTCTTGCAA30.10211027910142954No Hit
GTATAGTCATGATGCTAATGTCTCTGTATGAAAAAGCTGAATTGCAGTAA30.10211027910142954No Hit
CTCCATGGAGCAGGGACTTAAGCCTCCTCCAGCTGATGAAGTATGAAAGG30.10211027910142954No Hit
GTTTTATACCAGGGCTCATCCCTAATTAGCACAGATGCCTGAAGTATGTG30.10211027910142954No Hit
GTCATCCCGTATGGTTGGCTGTTAAAGCCATGAGCCAGGATCATCCCTGG30.10211027910142954No Hit
GCCGGGATGAGGTCACCCCGGCTGTCAGACACAGCCTTCCTCTGAGGACA30.10211027910142954No Hit
GTATTACCCTTTGAACTGGTTACTCTACTTCTGGGAGTGTGACACACAGA30.10211027910142954No Hit
TCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTCTG30.10211027910142954No Hit
GTCCTGCCCTATGGCCCGGGCTGCTGGAGTGCCCGTGCTCTGCCTTCACC30.10211027910142954No Hit
GTGTAAGTGGGATTTAGCCTCAACTAGACTAGTTCAGGCTGTGAACAGGG30.10211027910142954No Hit
GTCCAGAGGGCACACGTGGGCTTGCCACCAAGCACCCGGCCCTGAACTCG30.10211027910142954No Hit
GCCTAGACCTTCTGTCTTCCTACTAGTCTTTCTGATGTTGGTGCTTTGTT30.10211027910142954No Hit
GTGTTAGGTGCTGTGGGCAATCCAAGGGGCCTTCGACACCACCTTGCCTG30.10211027910142954No Hit
TGCATAGAGAGAGCACTCCAGAGAGACTAGGCTCTAGTCCAGGGAGGAGA30.10211027910142954No Hit
TCCTGGGACAGCCTAGACCTTCTGTCTTCCTACTAGTCTTTCTGATGTTG30.10211027910142954No Hit
ATGCTATGCTTTCACAGAGCTTTTCAGCTTGGCGCGAGCCGCACATCGCT30.10211027910142954No Hit
CCGTATGGTTGGCTGTTAAAGCCATGAGCCAGGATCATCCCTGGAAGAGA30.10211027910142954No Hit
GATCAAGGACTGAGCTCCTGGCACCATGAACCCCACCATTGGCTTCGCTC30.10211027910142954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACCT100.006732901145.08
AGGCTGA100.006732901145.05
GCTGACC100.006732901145.07
Produced by FastQC (version 0.11.5)