FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1982_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT50.17164435290078955No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG40.13731548232063165No Hit
CCTTTACAGTTACCGGAGACAACACCAAATGGAATGAGAATCAGAATCCT40.13731548232063165No Hit
GCTCAAGACATTTCTAAACCATTCAGGTTGGTTCCTTGTGATGTATGTTA40.13731548232063165No Hit
CCCGCTCAAAGGCAGGACTGTTGGTTTCAGATGGAGGGCCGAATTTATAC30.10298661174047373No Hit
GGACTAGGCCAGTGCCTTCCCCAGCCATGAGGAGACGCGAGAGGATCACT30.10298661174047373No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA30.10298661174047373No Hit
GTGTATCACCTCTGTGGCATCGGTAGGTGTATCTGTAGTCTTTAATGAAC30.10298661174047373No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA30.10298661174047373No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC30.10298661174047373No Hit
CTGTATACTTAACCAAAAATTGCTTTGACATTATGGAGAATTAAGAGGCC30.10298661174047373No Hit
CATTAATTGGCTTGTTTTCTTCATGTTCTTTGTTTTTGGGATTTTATCCT30.10298661174047373No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT30.10298661174047373No Hit
CTGTAACTCCAATGCTCATGTCAGCCGATTCATTAATTCCGGAAACTCCA30.10298661174047373No Hit
CCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACTAT30.10298661174047373No Hit
TCCCTGCTCCCAGCGGTCGAAATTCTTGCTGATGGATGCTCTGAAATTAA30.10298661174047373No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA30.10298661174047373No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)