FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1982_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCCTTTCTCAGGAAGCTATTAGAAATTGTGCTCCACTCAAAT50.20755500207555005No Hit
GTAATTATGTTCCTTCGAGTAATCTTTTCTCAATGACTATCCAGTGTACA40.16604400166044No Hit
CTCTTGTTTGTTGTCTTCCTGCCTACTTTTCTAAGAATTTTTCACTAATG40.16604400166044No Hit
GTGTCGAACGTTCCCAAAACATCACGCATCTGCTGGAATAGCGTTCTCAC40.16604400166044No Hit
GTTCCAGAGCTTGCTACTGCTAAGGGTAACCTTTGCCAGGTGATACCTGA30.12453300124533001No Hit
ATAAGATGGAGTTTGAACCATTTCAATCTTTAGTACCAAAGGCTGCCAGA30.12453300124533001No Hit
GAGAAAGACCAAGTAAAATGAGACCGGACAAGAGGGTTGAAGCTTTGGTG30.12453300124533001No Hit
GGTCAGGAGAGTGTTTGCTTCCTCTCTGCTCTCTAACGGGTGAAGATGCA30.12453300124533001No Hit
AGTTAACACATATCAATGGATCATTAGGAATTGGGAGACTGTAAAGATCC30.12453300124533001No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT30.12453300124533001No Hit
ATGTGATGTGATGGAAGTGTTGGCTAAGGCTACAGTAGCTATCAAATAGC30.12453300124533001No Hit
GTGTACATTATATGCAATTCCAAAGTTTCAAAGAAACTTTCTGCTGGCCT30.12453300124533001No Hit
TCCCCACACCGTTCACTGGAGGAGTGCCCTGACTCTTCATGGTGGGTATC30.12453300124533001No Hit
CCTCTATGGTGTTGGCCAAAGCAGTTGCAGCCGGCTGGTTTCTATTCAAT30.12453300124533001No Hit
GTATGGGCCCGGTGCCCGGCTCAGAGCGTGGCAGCAACAGTAACAGCTAG30.12453300124533001No Hit
GTATTTTCAGAGCCAGGAAGATCAATGCGTTCATGCATCATGAATTAGGC30.12453300124533001No Hit
GTTAAATGCAGGGGAGTTACCTCTCACAAGTATTCTCATTCCTGATCCCC30.12453300124533001No Hit
CTCCAGAAACATGCAGAGTTCCAGGGGCAAACACACTACACACATCCACT30.12453300124533001No Hit
CTTATTAGGTAGCTCTTTTTTGTCAGCTTTTGCTTCTTCTTCCCTATTGT30.12453300124533001No Hit
CCTATATTCCCATTGATGTAATATGAAGAAGTCCAGAGGAGGAGAGAGAG30.12453300124533001No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT30.12453300124533001No Hit
CTATACCTTCTTCTACTCACAAACTATGGACTCCAGCAAAGGACAAAACA30.12453300124533001No Hit
TCTATGATGTGGGAGATTAACGGGCCAGAATCAGTGCTAGTTAACACATA30.12453300124533001No Hit
GATGTACACACCAGGAGGGGAGGTAAGAAATGATGATGTTGACCAAAGTT30.12453300124533001No Hit
GTATACGTTAAACTTCACAAATGCTATATGTCAATTACAACTCAATTTAA30.12453300124533001No Hit
ATTTAGGTCTCACTGTGAGAATTATTTTGAGCTTCATCCTGTTCTGGAAA30.12453300124533001No Hit
CCTCTAAATCAGGGCGGCGGGGGGTAGCAGGTGCTCCCCAAGCACGGCAG30.12453300124533001No Hit
ATGTAAATGTTATTCAGTTCTGTAATACAGCATGCCATAATTAAACCTTT30.12453300124533001No Hit
GATATATGCAAGGCAGCAATGGGTTTGAAAATCAGCTCATCCTTCAGCTT30.12453300124533001No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)