FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1982-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169717
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGG1001.3916467E-436.249763145
CTTACGC1251.264216E-534.8042373
CCGTAGG1051.8507204E-434.533921
GCAATCG1102.436494E-432.954338
TACACCG1851.6422746E-731.355175
CGTTACA950.004724805230.5300312
AGTAGCG1100.00966650426.3668463
CGAAAGC8300.026.2080123
GTATAAG3607.2759576E-1226.1882211
TACACGG2101.5538953E-524.1696115
GCGAAAG9400.023.9124852
TAGTACC5750.022.6984164
TTACGCA1650.002599282721.9723724
CTGATCG2653.7810369E-621.886659
TATAGTA3355.4937118E-821.6444262
TACTATA3355.4937118E-821.6444262
GTATATA6400.020.3965951
GTACCTA5103.274181E-1119.9043856
AGCGAAA11200.019.425331
TAGGAGT5356.548362E-1118.9742724
Produced by FastQC (version 0.11.5)