FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1982-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628982
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTCG300.001935411472.499967
TATCGAA858.9195055E-751.17644145
AGCGAAA2400.039.2739331
GTCCTAT3800.034.344821
GCGAAAG3550.028.5915342
TATAGTG2156.1185347E-726.976735
TATACTG2456.430491E-826.6326395
GTTATAT2207.473409E-726.3657171
ATACTAC2601.143344E-725.096143
GTGTAAG3301.7680577E-924.1685751
GGTCGTT1801.5194161E-424.166655145
TATATAG2151.8643177E-523.604645
GTAATAC2151.8643177E-523.604643
GGTAGTC1851.8296219E-423.51358
CTATGAT5300.023.2547054
TATAAGA3854.274625E-1022.5973912
GTATATA2252.6479061E-522.5573351
TACTACA2603.2010448E-622.307684
TATACTA2603.2010448E-622.307682
GTATAGA4058.039933E-1021.4831771
Produced by FastQC (version 0.11.5)