FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1982-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987196
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC14660.14850141208027584No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGCGCGGCTGCGGCGTGG12870.13036924784946452No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG11730.11882138906559589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGA4500.040.27775145
GATATAT2750.034.2761761
GTACAAG2353.45608E-1133.9395831
GTATAAC2755.456968E-1231.6395451
TATACCG6050.031.1570035
TCATAGT4950.030.7575533
TGATCCG4550.030.2747069
GTATAAA6950.030.254841
TCGAAAC3251.8189894E-1228.999989
GTATATA3850.028.2495961
GTCATAG5150.028.155322
CCTATAG3900.027.8845942
CGAAAGC6700.027.052223
CGGTAGA2156.1190804E-726.9794581
ATTAGGT3005.875336E-1026.5833153
TCGGGTA3151.033186E-925.317444145
TATATAC5650.024.3805143
CCCTATA4500.024.1690981
GTAGGAC5400.024.166653
TATAGTC4201.8189894E-1224.166654
Produced by FastQC (version 0.11.5)