FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1982-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658456
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC1406.6050416E-736.258
ACGGTTC1051.8530303E-434.523808145
GCGAAAG3000.033.8333361
GTACGTC900.003627338432.222226
ACACGGT950.004726136630.5263146
TACACGT2054.03561E-728.2926837
CAACGCA1555.4976088E-528.0645169
AGCGAAA2603.7143764E-927.8846151
TATAGCC1306.48058E-427.8846153
TAGTACT1050.007706570427.6190474
TTGTACG1100.00966923326.3636364
GGATAAC1100.00966923326.3636361
CGAAAGC3751.0913936E-1125.1333352
TGCACCG1450.001225998225.05
CTGTACA3201.2387318E-924.9218754
CCTATAT2651.3748286E-724.622641
GATATAG1500.001493731724.1666681
GTACTGG1801.5195167E-424.1666661
ATACACG1851.8297425E-423.5135126
CCGACTC1550.001807835723.3870969
Produced by FastQC (version 0.11.5)