FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1982-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1982-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550461
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9640.17512593989401612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTT604.943222E-448.333157145
TGAATCG450.00963699448.3331535
GCGAAAG2150.040.4649662
AGCGAAA2005.456968E-1239.8965951
GTACGAA750.001478865738.6876071
ATACTAT951.0283112E-438.1577536
CGAAAGC2551.8189894E-1234.117523
TATACTG2850.033.0700535
GTAATAT1551.4572452E-632.759671
GTATAGG950.00471296930.542851
GTATAAG2401.5697879E-930.2246931
TGTAATA2802.6193447E-1028.4820372
CCTATAT2303.463356E-828.384931
TAAGCTA1050.007705630727.6189445
TTATACT1358.0782484E-426.8517514
TAATACT2808.179995E-925.8927614
GTCTAAT2301.0964195E-625.2310491
GTCCAAT1751.2501181E-424.8706051
CTGATCG1801.5191131E-424.1665769
GTAATAC2751.9653453E-723.7271843
Produced by FastQC (version 0.11.5)