FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1981_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGTCCCACAATTTTCCATCAACGCAAACAGAAACTTTGAGATCATT15.0No Hit
GCTCAGGCCCCTCACCTTGCCATTTAAAAGAAAACATTGCAGTCCAGGCC15.0No Hit
GAGTGTTCTAGAGAAACAGGCCCGTGGATGTGCAGGGCTGCTCTGGAGCT15.0No Hit
GGCTGGCACCGGTCCTCAGCCGAGACCCCGGCTCCCCCGGCCCGTCCTGC15.0No Hit
CTATGTTGTGGGATGGGCTGCCGTGAAGAAGGCTGAGAACATGCAGGATC15.0No Hit
CTCCTGAGCAAGTGGGACTTGATCCCAGGACCCACAAAATGTGACCTGAG15.0No Hit
ATATTTGGGTGAGGATTTTTATGTTTATGTTCATCACAGATACTTGGGAG15.0No Hit
GGCTGGTGCTCCTTTAGCTGGAAGCAGGTGAACTCTAGATGTAACTAACA15.0No Hit
GAGCGAAAGCAGGCGGAGTGAGGGAGGGTGAAGCAGGCTTCCTGCTGAGC15.0No Hit
GGAGATAACAGGAATGGTCTTCTACAAGCATGGGACTTTCAGAAACAACT15.0No Hit
CTTTTCACACAATGGCAGTCATGCTGCTTAGATGGTGAGTTCTAAACTTA15.0No Hit
GCTGGAAAGGCCCCTGAACACGCTTTCGCTCCCAGTCTCAAGTCCCTTCA15.0No Hit
GGAGGGAGCTGGCCCCCTTTCGACTGAACGTGGTTATTAGGAGAAGGTGG15.0No Hit
GGATACAGTCAGGACTCTAGACTGGTAGTTAGAATTATCACGTGTGGAAC15.0No Hit
GTATAAATGAACAAGGTACTAATAGCCTGTAGGATAGCTTTAAGTAATTT15.0No Hit
AAAGCAGGCAGAAGAAGAAGTAGGCTCCCTCCTGAGCAAGTGGGACTTGA15.0No Hit
CCTCATAATTAGCAGATTAAATTCATTAAACAACCAAAAGAAAAACATAA15.0No Hit
GGGCAGGGGACAAGCACCCCAGACCCCCGTGTCACCTGCCTTATCTCCAG15.0No Hit
GGGAAGTCCCTTTGCTAACTGTCCCTACCTTCTGTCCGCCTACACACACA15.0No Hit
GCACAGAGAAGTCCGAGGGGACTCGACCTCGGAACATCTTGGTGTCCTTG15.0No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)