FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1981_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624967
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1850.066.6375051
GGCCGAT400.006049867354.3749127
GCGAAAG2400.051.3540842
GTCATAC1151.4088073E-744.1409571
ATATAAG2109.094947E-1237.9761283
CGAAAGC3500.037.2856523
ACCGTAC1001.390866E-436.2499438
CAATCCG800.002036047936.2499439
GACGAGT850.00273834.1257821
CTATAGT950.004725991330.5262642
CCTATAA1255.144471E-429.0069141
GTATACG1000.00607278728.9999529
CTATAAC1306.480271E-427.8845672
GTCTATA1658.39998E-526.369921
CTCGTTG1100.00966894126.3635948
CGAGTGT1100.00966894126.3635943
TATAGGT1658.413709E-526.363594
CATACAC2852.7753777E-722.89473
TCGTTGT1600.00217426422.6562149
TCATACA2653.7762165E-621.8867552
Produced by FastQC (version 0.11.5)