FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1981_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784682
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9500.12106815244901756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT502.303208E-672.513611
AGCGAAA3150.041.4363441
GCGAAAG3350.038.957572
CGAAAGC3800.034.3441733
GTATTAG2054.0306622E-728.2979951
ATATAGC1958.747262E-626.0272086
GCTATAT2259.088435E-725.7826181
GTATTAC2559.463474E-825.5930391
GTATAAG1450.001224867225.004691
GTCTATA1851.82785E-423.517931
TATATAG2003.1009514E-421.7513085
TCTATAC8100.021.4827753
ATTGTAC1700.00309112821.3248143
TATACTG3106.2368235E-721.0496545
GTAATAT2806.0776347E-620.7181741
GTATAGG2104.307534E-420.7181741
TAATAAC1750.00365676320.7155323
GTAATAC1750.00365676320.7155323
TTACACT3157.2734474E-720.7155324
TACACTG2806.084343E-620.7155325
Produced by FastQC (version 0.11.5)