FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1981_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716835
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2700.053.7071652
CGAAAGC3000.048.336453
AGCGAAA3350.043.301481
CATAGCG855.3401403E-542.6468357
CTAATAC2003.250334E-729.0018713
GTATTAT5250.026.2489431
ATAGCGT1409.992128E-425.8927218
TATTATA3151.031367E-925.3190942
ATAATAC4600.025.2190173
GTCCATA3551.546141E-1024.5171741
ATTATAC2701.6464764E-724.1682243
GTATATA3952.1827873E-1123.8706231
TAGCGTG1600.002174620722.656139
TAATACA7200.022.1542054
TATTATC2653.7753325E-621.8882032
TATATAC3655.673428E-921.8507233
TATAATA4058.039933E-1021.4828662
TATATAA3809.025825E-920.9881942
TAGTACC2556.9505215E-519.9032444
TAGTACT2205.898562E-419.7740024
Produced by FastQC (version 0.11.5)