FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1981_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647221
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6730.10398302898082727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1951.6916601E-1037.1936571
CGTATCA800.002036138436.249805145
CCGACTA1051.8522124E-434.526294
CCGACCA950.004726230.5261549
GTATTAT6950.030.2633291
CCGTAGC1204.056607E-430.20817145
CGTGATC1255.152017E-428.9998448
TAGTACC2156.115133E-726.9786854
GTTACGT1100.0096511726.3736821
GCGAAAG2756.7502697E-926.3655342
CTATACA1751.2544436E-424.858934
TATAGAG1801.518736E-424.1684045
GTACCTA1851.8288032E-423.5152036
CGAAAGC3102.4127075E-823.3887793
GTGTTAG3753.0559022E-1023.2088431
GCTACTA1902.1920868E-422.8946159
GATATAA2053.655092E-421.2275981
CTATAGA2806.0810125E-620.7157764
TAGCATG8800.020.5980722
GTCCTAT9350.020.1681121
Produced by FastQC (version 0.11.5)