FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1981-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784873
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12240.15594879681171347No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11400.14524642840306648No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT9850.12549801050615833No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC9300.11849050738144896No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA8770.1117378225521836No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8550.10893482130229987No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC8240.10498513772291822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1950.066.93141
TTACGTG2200.059.321782
GCTACTA1457.2759576E-1249.9998449
CGTGTTG2800.046.609975
TACGTGT3050.042.7894823
GTGATCG1105.262964E-639.5453389
TATACTG3150.039.1293565
ACGTGTT3350.038.9575844
TTGTCGT750.001483214238.6665469
GTTATGT6050.035.9548841
GACGATG1801.2918281E-732.222122145
TAGCATG11650.031.7401222
GCATGTT11550.030.7594414
GTATATA4050.030.4358841
GGTTTAG2003.2492972E-729.0036071
GAGCGAA2003.2492972E-729.0036071
CTAGTAC1000.00607187229.0017593
TGTGTTG8050.028.8216235
TCAGGCG3301.8189894E-1228.5605189
GTTGACA7900.027.5350691
Produced by FastQC (version 0.11.5)