FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1981-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524262
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6510.12417455394440184No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC6500.1239838096219066No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5970.11387436052965882No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA5470.10433714440489679No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA5270.10052225795499196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG350.003564284462.148464
CCGACTA450.00963316948.337694
GGTCGAA450.009636848.3330767
TAATATA2350.046.2807662
GTAATAT2200.046.1493231
TATACTG2700.045.6522645
GTACAAG657.3232356E-444.627921
GTGATCG855.338168E-542.6468359
GAGTGCG1252.7157512E-740.5997857
GTATATA1553.4564437E-837.429871
TTATATG1656.002665E-835.1546862
TATATAG1051.8515356E-434.526923
CTTATAT1501.1300399E-633.842841
GGGATAC2603.6379788E-1233.46136145
CTATATA1153.158393E-431.524582
ACTATGT950.004725388330.5261548
TATATGG1703.006E-629.8556333
TAGTACC1000.006069184329.0026134
AGTGCGT2252.8117938E-828.9998478
GCGAAAG2553.0140654E-928.4339352
Produced by FastQC (version 0.11.5)