FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1981-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739125
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC30680.4150854050397429No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA17020.23027228141383393No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG15920.2153898190427871No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG14110.19090140368679182No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA12440.16830711990529343No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA12190.16492474209369187No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT11800.15964823270759346No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC10330.1397598511753763No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA10290.13921867072552005No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC9050.12244207677997632No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT8970.12135971588026383No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG8710.1178420429561982No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT8570.11594791138170132No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC8150.11026551665821072No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG8110.10972433620835446No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG8060.10904786064603415No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC7880.10661254862168103No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA7820.10580077794689667No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA7650.10350076103500762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTAT1700.055.463251
TTATACT5250.044.1961064
AGTAGAA3450.042.045721
TATACTA2600.041.835082
TATACTG5700.040.706945
CCTTATA5600.040.1417582
GCGAAAG2450.038.4768942
TATAGCG3700.037.236992
GTATAGG10950.037.092391
CGCTATA2950.036.8715972
CGAAAGC2650.035.5705683
GTCGGTA5350.035.233364145
TAATACA1458.6856744E-735.004464
ATAGGTG11550.034.528213
GCGGTAT4000.034.4418876
GGTACTG1707.809285E-834.1219945
ATAATAC850.002739813234.1219943
ACTATAC2800.033.6650053
CGGTATC4100.033.5972947
GTTATAC1956.9776434E-933.46583
Produced by FastQC (version 0.11.5)