FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1981-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708306
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3000.041.0832942
TATATAC3400.036.2499663
TATACTA950.004726398330.5262855
GTTATAC2151.8064384E-830.3530921
CGACTGT1204.056886E-430.2083036
GCTATAT1952.6022826E-729.747761
AGCGAAA3900.029.747761
CGAAAGC4300.028.6627623
GATATAC2303.4799086E-828.3735431
GTAGATC1100.00966976226.3636113
ATGCGCA1958.749845E-626.025616145
GTTATAG2301.1010088E-625.2209261
TAGGCTG3551.546141E-1024.5070195
ATTATAC1500.001493852424.1666433
TTAGTGC3052.0325388E-823.770474
TACTAGG1851.8299447E-423.513492
TATGCGG1600.002174540722.6562275
ACCGCGC1650.00260013221.9696778
ATATATA3901.2179953E-820.4486982
TATAAGA3208.463612E-720.3906042
Produced by FastQC (version 0.11.5)