FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1981-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1981-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508213
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCGG350.003565504762.1428153
CGTATAC606.800821E-660.416633
AAGGTCG400.006049196754.374969
CCTTACG450.00963645448.33332
CCGTATA450.00963645448.33332
TATACTG2002.237357E-1036.2499775
AGCGAAA2051.1352313E-831.8323781
GTATAGG2601.10958354E-1030.6760771
TTATAGT2451.9663275E-929.5918164
GCGAAAG2354.2888132E-827.7659382
TTATACT2104.978356E-727.619034
GGATAGC1907.1464146E-626.7105065
ACTAGTA1100.00966722826.3636198
TATAGGT3306.184564E-1126.3636172
TACATCG1450.001225617824.9999835
CTATTGT1450.001225617824.9999834
TATACTA1550.001807277723.3870815
TATACAG2202.2260247E-523.0681675
CTGATCC3803.619789E-1022.894729
GTATTAC1600.00217245422.6584641
Produced by FastQC (version 0.11.5)