FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1980-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1980-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552790
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6080.10998751786392663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTA1406.6027496E-736.249829
GTTGACA3850.033.9051361
TATACGT1204.05369E-430.2109172
GTATTAT6800.029.8608951
GTATACG1000.00606423929.0077291
TATAGAG1358.074083E-426.8541475
GGGTCGA1100.00966815126.3635069
ACATGGT5500.025.0475945
ATTAGGG1751.2540043E-424.8592683
TATAGAT1500.00149268624.1687322
ATAGTGG1801.5182054E-424.1687323
TATAATA3452.9540388E-923.1179182
ATATTAT2852.7670103E-722.9008371
GGATTAG2603.1924756E-622.3136351
TATTATT7800.022.3095992
TATTATC2603.197496E-622.3095972
CAGTGCA3955.875336E-1022.0271992
TATACTG1700.003089533421.3253525
CTACTGT3106.217615E-721.0539951
TGACATG6300.020.7160573
Produced by FastQC (version 0.11.5)