FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1980-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1980-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066800
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT22330.20931758530183725No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC20960.19647544056992874No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC12440.11661042369703788No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT12400.11623547056617922No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC12260.11492313460817398No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC12210.11445444319460067No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC11340.10629921259842519No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA10990.10301837270341206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTA608.032475E-872.49965145
TATACTG4250.046.0629165
GTAATAT3550.034.726281
GTGATCG1051.8538514E-434.5236439
AGCGAAA4200.032.805151
TATACTA1652.3849716E-630.760315
TACAGCC9000.030.6109627
CTATGCG3600.030.211024
TTAGGTA4850.028.4045854
ATAATAC11200.027.8372963
TAATATA5250.027.6215042
ATACTGT5850.027.267386
ATTAGGT4800.027.1899173
GAGTTCG1358.07887E-426.854244
TATAATA11650.026.7620332
CTATACT1907.1510804E-626.71294
TAATACA12850.026.5198674
GTATTAT7700.026.369691
GCGAAAG4950.026.3696881
CTATACA2259.1014044E-725.780074
Produced by FastQC (version 0.11.5)