FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1980-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1980-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789391
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG85811.0870405160433803No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC79741.0101457959363611No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG79201.0033050794853247No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA71440.9050014504852475No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGATCCAAA68910.8729514271127996No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT47310.5993227690713474No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA47110.5967891703857784No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG46300.5865280957092239No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA45530.5767737407697833No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT44060.5581517904308511No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG37170.47086931571299906No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC29770.3771261643469459No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA29580.3747192455956554No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA27560.34912989887140844No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT27540.34887653900285154No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG25760.32632751070128746No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC25720.32582079096417366No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC24590.31150595839070877No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG24180.30631208108529234No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGATCCAAATG22370.28338301298089286No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC21740.2754021771213505No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA20890.26463438270768225No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG19460.24651915210586386No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA19180.24297211394606727No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA19050.2413252748004474No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA18990.24056519519477676No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA18780.23790491657492926No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18080.22903732117543776No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT17580.22270332446151525No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA17210.21801616689321263No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG17210.21801616689321263No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT17080.21636932774759277No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG16860.21358236919346688No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT16810.2129489695220746No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA16380.20750173234810126No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA15850.20078769583134337No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG15740.19939421655428047No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG15670.1985074570143313No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA15410.1952137787230916No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG15390.19496041885453472No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA15210.1926801800375226No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT15040.19052662115478897No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG15030.1903999412205105No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG14950.18938650174628288No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG14910.1888797820091691No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA14850.1881197024034984No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT14850.1881197024034984No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG14690.1860928234550432No Hit
AAATAGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTT14610.18507938398081558No Hit
CTGCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCAT14560.18444598430942336No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT14470.18330586490091727No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT14340.18165902575529744No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTTGCAGATGC14120.17887206720117152No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT13870.17570506884421028No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG13690.1734248300271982No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC13580.17203135075013523No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC13530.17139795107874298No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG13200.16721751324755413No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA13100.16595071390476962No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA12820.16240367574497303No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA12760.16164359613930232No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA12750.16151691620502387No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC12710.16101019646791007No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG12600.15961671719084713No Hit
AAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTT12560.1591099974537333No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA12540.15885663758517643No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12470.15796987804522727No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGATCCAAATGCATTGTTGAAG12380.15682975863672122No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT12330.15619635896532896No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA12250.15518291949110138No Hit
GTTGAAGCACAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACA12100.15328272047692462No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG12080.15302936060836772No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC12030.15239596093697547No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT12000.15201592113414011No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC11940.1512558415284694No Hit
CAACAGAACACATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAG11830.14986236225140648No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT11670.14783548330295126No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG11560.1464420040258883No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT11520.14593528428877453No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG11490.14555524448593918No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG11440.1449218448145469No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC11440.1449218448145469No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA11290.14302164580037016No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA11270.14276828593181326No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT11230.14226156619469946No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC11000.13934792770629512No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG10990.13922124777201664No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT10880.1378277684949537No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT10840.1373210487578399No Hit
CCCCAACTCAACCCTATTGATGGACCATTACCTGAAGACAATGAGCCGAG10690.13542084974366317No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA10560.1337740105980433No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG10500.1330139309923726No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT10440.1322538513867019No Hit
GTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCT10350.13111373197819584No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG10220.12946689283257598No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG10160.1287068132269053No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT10020.126933294147007No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG9890.12528645500138716No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA9870.12503309513283023No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT9690.12275285631581814No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA9580.1213593770387552No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAGCAATGGCCT9390.11895245828746463No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC9380.11882577835318618No Hit
ATTCACCACTGTCCAGGCCATTGCTCAGCTGAAAATCCTTCGAGTTGTGG9360.1185724184846293No Hit
ACCCTATTGATGGACCATTACCTGAAGACAATGAGCCGAGCGGGTATGAT9320.11806569874751548No Hit
GAATATAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCTT9310.11793901881323704No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA9270.11743229907612324No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC9150.11591213986478184No Hit
CTTCCATTTCATTGATCCAAATGCATTGTTGAAGCACAAGGTGTGCAGAA9100.11527874019338959No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT8990.11388526091632664No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA8960.11350522111349129No Hit
CATCAATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCA8880.11249178163926368No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA8870.11236510170498525No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT8870.11236510170498525No Hit
AGCACAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA8840.1119850619021499No Hit
GAGTAGTAGTTTTCTCGATTTGCAGATGCTATTCACCACTGTCCAGGCCA8820.11173170203359298No Hit
GAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGCACCCCAACTCAA8690.11008486288797313No Hit
CTCGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTC8660.10970482308513779No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT8600.10894474347946709No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT8580.10869138361091017No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC8560.10843802374235328No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA8520.1079313040052395No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC8500.10767794413668258No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA8500.10767794413668258No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8490.10755126420240414No Hit
GATCCAAATGCATTGTTGAAGCACAAGGTGTGCAGAACCTTACTAGCAAA8370.10603110499106272No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG8360.10590442505678428No Hit
ACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAG8350.10577774512250585No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT8290.10501766551683513No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT8290.10501766551683513No Hit
GTCCAGGCCATTGCTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAATAT8220.10413090597688598No Hit
ATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGG8170.10349750630549372No Hit
CTTCTGGTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCTAGTGTTGA8060.10210402702843077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAA100.0070907567144.99888
CGCACCG100.0070907567144.9988145
AGTAGAA10050.0112.56491
CGCAATT203.8658793E-4108.7491145
GCAGGTA11050.0106.3089142
CAGGTAC12100.097.083763
GGTACTG12050.096.884825
GGTCAAA2200.095.591611
GACTTAA259.38632E-486.999275145
GCGAAAG2100.082.87222
CGAAAGC2150.080.944953
GTACTGA14550.080.2379466
ATTGTTA755.638867E-1177.33269145
AGAAACA14950.076.150964
AGCAGGT15850.073.661581
AGTTTTA702.8121576E-972.499405145
CTGATCC16200.072.4994059
GCCTGCC502.3059038E-672.4994145
ACTGATC17000.072.072948
AAATAGA3350.071.435421
Produced by FastQC (version 0.11.5)