FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1980-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1980-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954016
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC43410.45502381511421197No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA23880.2503102673330426No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG23300.2442307047261262No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC22990.2409812833327743No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA22190.23259567973702744No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT20510.21498591218595914No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG18600.1949652836011136No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT18050.18920018112903766No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC17930.18794234058967563No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC16360.1714855935330225No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG15450.1619469694428605No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC14860.15576258679099722No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG14460.15156978499312382No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC14340.15031194445376178No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG14270.14957820413913392No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC14020.14695770301546304No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC13830.1449661221614732No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA13640.1429745413074833No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA13530.14182152081306812No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG12720.13333109717237449No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA12720.13333109717237449No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC12650.13259735685774662No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT12440.13039613591386306No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT12020.125993694026096No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA11360.11907557105960487No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA11130.11666471002582765No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT10920.11446348908194413No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT10750.11268154831784792No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT10650.11163334786837957No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT10640.11152852782343273No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT10510.11016586723912387No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC10490.1099562271492302No Hit
GCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGG10450.10953694696944287No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC10370.10869838660986818No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA10270.10765018616039983No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG10140.10628752557609096No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT10020.10502968503672894No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA9970.10450558481199478No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA9910.10387666454231374No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG9880.10356220440747325No Hit
CAGATACACCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGAA9790.10261882400295172No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT9770.10240918391305806No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG9690.10157062355348337No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG9550.10010314292422769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA301.1016709E-7120.8332063
TCGTGCC203.8660184E-4108.749886145
TAGTATA203.8660184E-4108.749886145
GATAACG352.7590795E-7103.58761
ACGAATA352.7616625E-7103.571325
AGTAGAA3100.093.5629961
GCAGGTA1450.084.99992
TAGATAA353.4372144E-582.8570562
CTTAACT406.663066E-572.499924
GGTACTG1600.072.499925
TGGATTA300.00193572372.49992145
CAGGGTG903.6379788E-1272.4999165
CGCAATT554.0614887E-665.90902145
GGCCCAC350.003566468862.142797
AACACTG4950.058.5857965
ATATCAC1550.056.1289753
GACAGTA4800.054.3749437
CAACCCG400.006050826554.374943145
CTTATAA400.006050826554.374943145
TAACTAT4500.051.5636021
Produced by FastQC (version 0.11.5)