FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1980-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1980-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555765
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC16160.29077037956690327No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA9270.1667971174867075No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA8850.15923996653261718No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG8680.15618111971786636No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG7930.14268620729984796No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC7330.13189027736543324No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG6730.1210943474310185No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT6550.11785556845069409No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC6260.1126375356490603No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT6070.10921882450316231No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG6060.10903889233758873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT406.110622E-790.63311
GGTACTG1950.059.487145
AGGTACT2650.049.245254
TTGATCG450.009636925548.3333145
TTACGTG803.7325117E-545.3124732
TACGTGT803.7325117E-545.3124733
CAGGTAC2800.044.017833
TATACTA2500.043.4999732
GCGAAAG1201.971348E-742.291642
GTACTGA2750.042.181796
GACAGTA3250.040.153827
GTATAGG8550.039.0093351
GCAGGTA3450.037.826062
TAACTAT2151.2732926E-1137.0963361
TTATATC1001.3906593E-436.2499774
TACCTAT1001.3906593E-436.2499777
CTACTAG3100.035.080626
CTAGTAG3000.033.8333139
ACCGTGT2154.8567017E-1033.720918
AGCACCG1956.9812813E-933.4615175