Basic Statistics
Measure | Value |
---|---|
Filename | HK7MWAFX2_n01_1980-d02-rep2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 555765 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC | 1616 | 0.29077037956690327 | No Hit |
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA | 927 | 0.1667971174867075 | No Hit |
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA | 885 | 0.15923996653261718 | No Hit |
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG | 868 | 0.15618111971786636 | No Hit |
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG | 793 | 0.14268620729984796 | No Hit |
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC | 733 | 0.13189027736543324 | No Hit |
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG | 673 | 0.1210943474310185 | No Hit |
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT | 655 | 0.11785556845069409 | No Hit |
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC | 626 | 0.1126375356490603 | No Hit |
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT | 607 | 0.10921882450316231 | No Hit |
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG | 606 | 0.10903889233758873 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTACGT | 40 | 6.110622E-7 | 90.6331 | 1 |
GGTACTG | 195 | 0.0 | 59.48714 | 5 |
AGGTACT | 265 | 0.0 | 49.24525 | 4 |
TTGATCG | 45 | 0.0096369255 | 48.3333 | 145 |
TTACGTG | 80 | 3.7325117E-5 | 45.312473 | 2 |
TACGTGT | 80 | 3.7325117E-5 | 45.312473 | 3 |
CAGGTAC | 280 | 0.0 | 44.01783 | 3 |
TATACTA | 250 | 0.0 | 43.499973 | 2 |
GCGAAAG | 120 | 1.971348E-7 | 42.29164 | 2 |
GTACTGA | 275 | 0.0 | 42.18179 | 6 |
GACAGTA | 325 | 0.0 | 40.15382 | 7 |
GTATAGG | 855 | 0.0 | 39.009335 | 1 |
GCAGGTA | 345 | 0.0 | 37.82606 | 2 |
TAACTAT | 215 | 1.2732926E-11 | 37.096336 | 1 |
TTATATC | 100 | 1.3906593E-4 | 36.249977 | 4 |
TACCTAT | 100 | 1.3906593E-4 | 36.249977 | 7 |
CTACTAG | 310 | 0.0 | 35.08062 | 6 |
CTAGTAG | 300 | 0.0 | 33.833313 | 9 |
ACCGTGT | 215 | 4.8567017E-10 | 33.72091 | 8 |
AGCACCG | 195 | 6.9812813E-9 | 33.461517 | 5 |